DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) -
Detection method
Fluorescent -
Product overview
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) (ab238535) is an assay for measuring the total free radical presence of a sample.
The assay employs a proprietary quenched fluorogenic probe, dichlorodihydrofluorescin DiOxyQ (DCFH-DiOxyQ), which is a specific ROS/RNS probe that is based on similar chemistry to the popular 2’, 7’-dichlorodihydrofluorescein diacetate. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2’, 7’-dichlorodihydrofluorescein (DCF). Fluorescence intensity is proportional to the total ROS/RNS levels within the sample. The DCFH-DiOxyQ probe can react with hydrogen peroxide (H2O2), peroxyl radical (ROO·), nitric oxide (NO), and peroxynitrite anion (ONOO-).
The kit has a detection sensitivity limit of 10 pM for DCF and 40 nM for H2O2 respectively. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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Notes
Related products
Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
To measure reactive oxygen species within cells, we recommend DCFDA / H2DCFDA - Cellular ROS Assay Kit ab113851. Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029). ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates.
For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests Catalyst (250X) 1 x 20µl DCF Standard 1 x 100µl DCF-DiOxyQ 1 x 50µl Hydrogen Peroxide 1 x 100µl Priming Reagent 1 x 250µl Stabilization Solution (10X) 1 x 1.5ml
Images
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab238535 has been referenced in 10 publications.
- Xiao L et al. IL-9/STAT3/fatty acid oxidation-mediated lipid peroxidation contributes to Tc9 cell longevity and enhanced antitumor activity. J Clin Invest 132:N/A (2022). PubMed: 35192544
- Tan Y et al. Trichosanthin inhibits cell growth and metastasis by promoting pyroptosis in non-small cell lung cancer. J Thorac Dis 14:1193-1202 (2022). PubMed: 35572907
- Fu S et al. Insulin-incubated palladium clusters promote recovery after brain injury. J Nanobiotechnology 20:299 (2022). PubMed: 35752849
- Alsuliam SM et al. Fenugreek Seed Galactomannan Aqueous and Extract Protects against Diabetic Nephropathy and Liver Damage by Targeting NF-?B and Keap1/Nrf2 Axis. Toxics 10:N/A (2022). PubMed: 35878267
- Choudhury D et al. Inhibition of glutaminolysis restores mitochondrial function in senescent stem cells. Cell Rep 41:111744 (2022). PubMed: 36450260
- Vandierendonck A et al. NOX1 inhibition attenuates the development of a pro-tumorigenic environment in experimental hepatocellular carcinoma. J Exp Clin Cancer Res 40:40 (2021). PubMed: 33485364
- Cao JY et al. Autophagosome protects proximal tubular cells from aldosterone-induced senescence through improving oxidative stress. Ren Fail 43:556-565 (2021). PubMed: 33757397
- Jin L & Tong L PAQR3 inhibits proliferation and aggravates ferroptosis in acute lymphoblastic leukemia through modulation Nrf2 stability. Immun Inflamm Dis 9:827-839 (2021). PubMed: 33955706
- Lv Z et al. Identifying a Ferroptosis-Related Gene Signature for Predicting Biochemical Recurrence of Prostate Cancer. Front Cell Dev Biol 9:666025 (2021). PubMed: 34778244
- Tsao SP et al. Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson's Disease Rats. Antioxidants (Basel) 10:N/A (2021). PubMed: 34829694