DCFA (5(6)-Carboxy-2',7'-dichlorofluorescein), Amine-reactive green fluorophore (ab145439)

Overview

  • Product name

    DCFA (5(6)-Carboxy-2',7'-dichlorofluorescein), Amine-reactive green fluorophore
  • Description

    Amine-reactive, green fluorophore. Intracellular pH indicator in the pH 4-5 range.
  • Biological description

    Amine-reactive, green fluorophore. Intracellular pH indicator in the pH 4-5 range. DFCA DA (ab145415) precursor. 2,7-Dichlorofluorescein (ab146332) derivative.

  • Purity

    > 95%
  • CAS Number

    111843-78-8
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    529nm
  • Chemical name

    5(6)-Carboxy-2',7'-dichlorofluorescein
  • Molecular weight

    445.21
  • Molecular formula

    C21H10Cl2O7
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Ellis LC  et al. Quantification of biliary excretion and sinusoidal excretion of 5(6)-carboxy-2',7'-dichlorofluorescein (CDF) in cultured hepatocytes isolated from Sprague Dawley, Wistar and Mrp2-deficient Wistar (TR(-)) rats. Toxicol In Vitro 28:1165-75 (2014). Read more (PubMed: 24907646) »
  • Pogue AI  et al. Metal-Sulfate Induced Generation of ROS in Human Brain Cells: Detection Using an Isomeric Mixture of 5- and 6-Carboxy-2',7'-Dichlorofluorescein Diacetate (Carboxy-DCFDA) as a Cell Permeant Tracer. Int J Mol Sci 13:9615-26 (2012). Read more (PubMed: 22949820) »
  • Nedergaard M  et al. Dicarboxy-dichlorofluorescein: a new fluorescent probe for measuring acidic intracellular pH. Anal Biochem 187:109-14 (1990). Read more (PubMed: 2372106) »

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