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    dck-antibody-ab151966.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-DCK antibody (ab151966)

  • Datasheet
  • SDS
Submit a review Submit a question References (7)

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Western blot - Anti-DCK antibody (ab151966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab151966)

Key features and details

  • Rabbit polyclonal to DCK
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human DCK protein (ab173027)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DCK antibody
    See all DCK primary antibodies
  • Description

    Rabbit polyclonal to DCK
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-260 of Human DCK (Uniprot ID#P27707)

  • Positive control

    • 293T, A431, HeLa or HepG2 whole cell lysate (ab7900), Saos2 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human DCK protein (ab173027)
  • Related Products

    • Recombinant Human DCK protein (ab99520)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 30 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 30 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similarities

    Belongs to the DCK/DGK family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P27707 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1633 Human
    • Omim: 125450 Human
    • SwissProt: P27707 Human
    • Unigene: 709 Human
    • Alternative names

      • dCK antibody
      • DCK protein antibody
      • DCK_HUMAN antibody
      • Deoxycytidine kinase antibody
      • Deoxynucleoside kinase antibody
      • EC 2.7.1 antibody
      • EC 2.7.1.74 antibody
      • MGC117410 antibody
      • MGC138632 antibody
      • OTTHUMP00000219118 antibody
      • OTTHUMP00000219119 antibody
      see all

    Images

    • Western blot - Anti-DCK antibody (ab151966)
      Western blot - Anti-DCK antibody (ab151966)
      All lanes : Anti-DCK antibody (ab151966) at 1/10000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 30 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab151966)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab151966)
      Immunohistochemical analysis of paraffin embedded Human Saos2 xenograft tissue labeling cytosolic DCK with ab151966 at a 1/500 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab151966? Please let us know so that we can cite the reference in this datasheet.

    ab151966 has been referenced in 7 publications.

    • Zhao Y  et al. Gemcitabine resistance in triple-negative breast cancer cells can be reverted by Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol. Oncol Lett 20:247 (2020). PubMed: 32973960
    • Zhang Y  et al. Role of Damage DNA-Binding Protein 1 in Pancreatic Cancer Progression and Chemoresistance. Cancers (Basel) 11:N/A (2019). PubMed: 31842285
    • Patel GK  et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). PubMed: 28152544
    • Cruickshank MN  et al. Systematic chemical and molecular profiling of MLL-rearranged infant acute lymphoblastic leukemia reveals efficacy of romidepsin. Leukemia 31:40-50 (2017). PubMed: 27443263
    • Shan J  et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). WB ; Human . PubMed: 27471030
    • Huang YH  et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget 7:21812-24 (2016). PubMed: 26943573
    • Wang C  et al. Establishment of human pancreatic cancer gemcitabine-resistant cell line with ribonucleotide reductase overexpression. Oncol Rep 33:383-90 (2015). PubMed: 25394408

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