Overview

  • Product name

  • Description

    Rabbit polyclonal to DCK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-260 of Human DCK (Uniprot ID#P27707)

  • Positive control

    • 293T, A431, HeLa or HepG2 whole cell lysate (ab7900), Saos2 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similarities

    Belongs to the DCK/DGK family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Images

  • All lanes : Anti-DCK antibody (ab151966) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human Saos2 xenograft tissue labeling cytosolic DCK with ab151966 at a 1/500 dilution.

References

This product has been referenced in:

  • Cruickshank MN  et al. Systematic chemical and molecular profiling of MLL-rearranged infant acute lymphoblastic leukemia reveals efficacy of romidepsin. Leukemia 31:40-50 (2017). Read more (PubMed: 27443263) »
  • Patel GK  et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). Read more (PubMed: 28152544) »
See all 5 Publications for this product

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