Overview

  • Product name

  • Description

    Rabbit polyclonal to DCK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 - 216 of Human DCK.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab96599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similarities

    Belongs to the DCK/DGK family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Images

  • Anti-DCK antibody (ab96599) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS-PAGE
  • ab96599, at 1/200 dilution, staining DCK in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

References

This product has been referenced in:

  • Hu Q  et al. dCK negatively regulates the NRF2/ARE axis and ROS production in pancreatic cancer. Cell Prolif 51:e12456 (2018). Read more (PubMed: 29701272) »
  • Hammam K  et al. Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology. Nat Commun 8:1420 (2017). Read more (PubMed: 29127277) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful so far. However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.
The details provided in the questionnaire enable us to closely monitor the quality of our products.
As requested, I have issued a free of charge replacement
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification
murine pancreatic adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kris Frese

Verified customer

Submitted Jan 23 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

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