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o echnique does not require any optimization and it works very well with all the other antibodies we have, (including abcam) we tried some of your suggestions but without any successful result.
Increasing dilution of the secondary are not decreasing the unspecifity but only increasing the time of exposure to get visible bands.
Our secondary antibody does not create any unspecific binding with any other antibody it is Affinity purified goat anti-rabbit IgG (H&L) antibody conjugated to horseradish peroxidase.
At this point we have already wasted too much time and a lot of other reagents to run blots with different conditions which have shown unsuccessful results to the antibody.
We would like to receive these two substitutions as soon as possible.
Asked on May 29 2012
rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful so far. However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.
The details provided in the questionnaire enable us to closely monitor the quality of our products.
As requested, I have issued a free of charge replacement
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on May 29 2012