Key features and details
- Mouse monoclonal [OTI16E12] to DCK
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-DCK antibody [OTI16E12]
See all DCK primary antibodies
DescriptionMouse monoclonal [OTI16E12] to DCK
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human DCK aa 1-260. (NP_000779) produced in HEK293T cells.
MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEV VPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYA CLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNET EWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGI PLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLV EKVKEFLSTL
Database link: P27707
- WB: HEK293T cells transfected with pCMV6-ENTRY DCK. Flow: HEK-293T transfected with DCK, HeLa and Jurkat cells.
The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab118994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 31 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionRequired for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Sequence similaritiesBelongs to the DCK/DGK family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
ab118994 has not yet been referenced specifically in any publications.