Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
Key features and details
- Mouse monoclonal [OTI16G6] to DCK - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DCK antibody [OTI16G6] - BSA and Azide free
See all DCK primary antibodies -
Description
Mouse monoclonal [OTI16G6] to DCK - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human DCK aa 1-300.
Database link: P27707 -
Positive control
- WB: pCMV6-ENTRY DCK transfected HEK-293T lysate; HepG2, HeLa, Jurkat cell lysate, human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland and colon tissue lysate; IHC-P: Human breast adenocarcinoma, lung carcinoma, bladder carcinoma, lymph node, lymphoma tissue; Flow Cyt (Intra): HeLa and Jurkat whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI16G6 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
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|
WB |
1/500 - 1/2000. Predicted molecular weight: 31 kDa.
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|
IHC-P |
1/150.
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Notes |
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Flow Cyt (Intra)
1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 31 kDa. |
IHC-P
1/150. |
Target
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Function
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
Sequence similarities
Belongs to the DCK/DGK family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1633 Human
- Omim: 125450 Human
- SwissProt: P27707 Human
- Unigene: 709 Human
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Alternative names
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
Images
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All lanes : Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650) at 1/2000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human omentum tissue lysate
Lane 3 : Human uteus tissue lysate
Lane 4 : Human breast tissue lysate
Lane 5 : Human brain tissue lysate
Lane 6 : Human liver tissue lysate
Lane 7 : Human ovary tissue lysate
Lane 8 : Human thyroid tissue lysate
Lane 9 : Human colon tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining DCK with a mouse monoclonal
DCK antibody [OTI16G6].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using a mouse monoclonal DCK antibody [OTI16G6] (Red), compared to a nonspecific negative control antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma tissue staining DCK with a mouse monoclonal
DCK antibody [OTI16G6].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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All lanes : Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY DCK-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using a mouse monoclonal DCK antibody [OTI16G6] (Red), compared to a nonspecific negative control antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue staining DCK with a mouse monoclonal
DCK antibody [OTI16G6].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Immunohistochemical analysis of paraffin-embedded human lymph node tissue staining DCK with a mouse monoclonal
DCK antibody [OTI16G6].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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Immunohistochemical analysis of paraffin-embedded human lymphoma tissue staining DCK with a mouse monoclonal
DCK antibody [OTI16G6].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab273650 has not yet been referenced specifically in any publications.