Product nameAnti-Dcp1a antibody
See all Dcp1a primary antibodies
DescriptionRabbit polyclonal to Dcp1a
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse, Cat, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region within amino acids 532-582 of Human Dcp1a (NP_060873.4).
- Hela, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab128156 was affinity purified using an epitope specific to Dcp1a immobilized on solid support.
Our Abpromise guarantee covers the use of ab128156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 63 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Tissue specificityDetected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
Sequence similaritiesBelongs to the DCP1 family.
Cellular localizationCytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
- Information by UniProt
- DCP1 decapping enzyme homolog A antibody
- Dcp1a antibody
- DCP1A_HUMAN antibody
All lanes : Anti-Dcp1a antibody (ab128156) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 3 minutes
Detection of Dcp1a by Western Blot of Immunprecipitate.
ab128156 at 1 µg/ml staining Dcp1a in HeLa whole cell lysate immunoprecipitated using ab128156 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. Predicted band size: 63 kDa.
ab128156 has not yet been referenced specifically in any publications.