Anti-Dcp1a antibody (ab128169)

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Overview

  • Product name

    Anti-Dcp1a antibody
    See all Dcp1a primary antibodies

  • Description

    Rabbit polyclonal to Dcp1a

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 225-275 of Human Dcp1a (NP_060873.4).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab128169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

  • Tissue specificity

    Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

  • Sequence similarities

    Belongs to the DCP1 family.

  • Cellular localization

    Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt

  • Database links

    • Alternative names

      • DCP1 decapping enzyme homolog A antibody
      • Dcp1a antibody
      • DCP1A_HUMAN antibody
      • Decapping enzyme hDcp1a antibody
      • Decapping mRNA 1A antibody
      • HSA275986 antibody
      • mRNA decapping enzyme 1A antibody
      • mRNA-decapping enzyme 1A antibody
      • Nbla00360 antibody
      • Putative protein product of Nbla00360 antibody
      • Smad4 interacting transcriptional co activator antibody
      • Smad4-interacting transcriptional co-activator antibody
      • Smad4-interacting transcriptional co-activator antibody
      • SMAD4IP1 antibody
      • SMIF antibody
      • Transcription factor SMIF antibody
      see all

    Images

    • Western blot - Anti-Dcp1a antibody (ab128169)
      Western blot - Anti-Dcp1a antibody (ab128169)
      All lanes : Anti-Dcp1a antibody (ab128169) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 63 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-Dcp1a antibody (ab128169)
      Immunoprecipitation - Anti-Dcp1a antibody (ab128169)
      Detection of Dcp1a by Western Blot of Immunprecipitate.
      ab128169 at 1 µg/ml staining Dcp1a in HeLa whole cell lysate immunoprecipitated using ab128169 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 63 kDa.
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab128169 has not yet been referenced specifically in any publications.

    Publishing research using ab128169? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    ChIP abreview for Anti-Dcp1a antibody

     Poor
    Abreviews
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Human Cell lysate - nuclear (HCT-116 colon cancer cell line)
    Specification
    HCT-116 colon cancer cell line
    Negative control
    IgG
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Positive control
    none, we don't have any antibody that works for Dcp1a in ChIP, so, there is no control.
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 08 2014

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