Overview

  • Product name

  • Description

    Rabbit polyclonal to Dcp1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 225-275 of Human Dcp1a (NP_060873.4).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab128169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • Tissue specificity

    Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • Sequence similarities

    Belongs to the DCP1 family.
  • Cellular localization

    Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

Images

  • All lanes : Anti-Dcp1a antibody (ab128169) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 63 kDa


    Exposure time: 30 seconds
  • Detection of Dcp1a by Western Blot of Immunprecipitate.
    ab128169 at 1 µg/ml staining Dcp1a in HeLa whole cell lysate immunoprecipitated using ab128169 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 63 kDa.
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab128169 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - nuclear (HCT-116 colon cancer cell line)
Specification
HCT-116 colon cancer cell line
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
none, we don't have any antibody that works for Dcp1a in ChIP, so, there is no control.

Abcam user community

Verified customer

Submitted Apr 08 2014

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