Key features and details
- Rabbit polyclonal to Dcp1a
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dcp1a antibody
See all Dcp1a primary antibodies
DescriptionRabbit polyclonal to Dcp1a
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 225-275 of Human Dcp1a (NP_060873.4).
- HeLa, 293T and Jurkat whole cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab128169 was affinity purified using an epitope specific to Dcp1a immobilized on solid support.
Our Abpromise guarantee covers the use of ab128169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 63 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Tissue specificityDetected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
Sequence similaritiesBelongs to the DCP1 family.
Cellular localizationCytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
- Information by UniProt
- DCP1 decapping enzyme homolog A antibody
- Dcp1a antibody
- DCP1A_HUMAN antibody
All lanes : Anti-Dcp1a antibody (ab128169) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 30 seconds
Detection of Dcp1a by Western Blot of Immunprecipitate.
ab128169 at 1 µg/ml staining Dcp1a in HeLa whole cell lysate immunoprecipitated using ab128169 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 63 kDa.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab128169 has not yet been referenced specifically in any publications.