Anti-Dcp1a antibody (ab47811)
- Datasheet
- References (15)
- Protocols
Overview
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Product nameAnti-Dcp1a antibody
See all Dcp1a primary antibodies -
DescriptionRabbit polyclonal to Dcp1a
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Dcp1a.
Read Abcam's proprietary immunogen policy (Peptide available as ab71605.) -
Positive control
- This antibody gave a positive signal in the following Human Whole cell Lysates: HeLa, Jurkat and HepG2 This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa). Abcam recommends using milk as the blocking agent. |
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| ICC/IF | 1/800. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
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Tissue specificityDetected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
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Sequence similaritiesBelongs to the DCP1 family.
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Cellular localizationCytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
- Information by UniProt
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Database links
- Entrez Gene: 55802 Human
- Omim: 607010 Human
- SwissProt: Q9NPI6 Human
- Unigene: 476353 Human
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Alternative names
- DCP1 decapping enzyme homolog A antibody
- Dcp1a antibody
- DCP1A_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody (ab47811)This image is courtesy of an Abreview submitted by Dr Kirk McManusab47811 (1/800) stainning Dcp1a in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody (ab47811)IHC image of Dcp1a staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47811, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Dcp1a antibody (ab47811)All lanes : Anti-Dcp1a antibody (ab47811) at 1 mg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) WCL at 10 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) WCL
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) WCL
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)
Exposure time: 12 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab47811 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunoprecipitation - Anti-Dcp1a antibody (ab47811)Dcp1a was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47811.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 75kDa: Dcp1a.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hernández G et al. Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Nat Commun 9:967 (2018). Read more (PubMed: 29511213) »
- Wu C et al. Overexpression of mRNA-decapping enzyme 1a affects survival rate in colorectal carcinoma. Oncol Lett 16:1095-1100 (2018). Read more (PubMed: 29963186) »
