Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab47998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Tissue specificityDetected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
Sequence similaritiesBelongs to the DCP1 family.
Cellular localizationCytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
- Information by UniProt
- DCP1 decapping enzyme homolog A antibody
- Dcp1a antibody
- DCP1A_HUMAN antibody
Anti-Dcp1a antibody (ab47998) at 2 µg/ml + A431 cell lysate in RIPA buffer at 35 µg
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible non-specific binding)
Primary incubation was 1 hour. Detected by chemiluminescence.
ab47998 has not yet been referenced specifically in any publications.