Product nameAnti-Dcp2 antibody
See all Dcp2 primary antibodies
DescriptionRabbit polyclonal to Dcp2
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, EMSA, IPmore details
Species reactivityReacts with: Mouse, Human
- This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293. This antibody gave a positive signal in the following Mouse whole cell lysate: NIH 3T3 (data not shown).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa).|
|IHC-P||1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|EMSA||Use at an assay dependent concentration. PubMed: 20691152|
|IP||Use at an assay dependent concentration.|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
Sequence similaritiesBelongs to the Nudix hydrolase family. DCP2 subfamily.
Contains 1 nudix hydrolase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
- Information by UniProt
- DCP2 antibody
- DCP2 decapping enzyme homolog (S. cerevisiae) antibody
- DCP2, S. cerevisiae, homolog of antibody
All lanes : Anti-Dcp2 antibody (ab28658) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human Dcp2 peptide (ab30458) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 48.4 kDa
Observed band size: 52,58 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Dcp2 contains a number of potential phosphorylation sites (Swiss Prot data) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas
ab28658 stainning Dcp2 in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Dcp2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28658.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52,58kDa: Dcp2.
This product has been referenced in:
- Seto E et al. The Assembly of EDC4 and Dcp1a into Processing Bodies Is Critical for the Translational Regulation of IL-6. PLoS One 10:e0123223 (2015). WB . Read more (PubMed: 25970328) »
- Wang X et al. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature 505:117-20 (2014). WB . Read more (PubMed: 24284625) »