The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.
Use a concentration of 5 - 20 µg/ml.
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
Belongs to the Nudix hydrolase family. DCP2 subfamily. Contains 1 nudix hydrolase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.