Overview

  • Product name

  • Description

    Rabbit polyclonal to Dcp2/TDT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Dcp2/TDT aa 1-160.
    Database link: Q8IU60

  • Positive control

    • HeLa xenograft tissue; NT2D1, IMR32, U-87MG and MCF7 whole cell lysates.
  • General notes

     This product was previously labelled as Dcp2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
  • Sequence similarities

    Belongs to the Nudix hydrolase family. DCP2 subfamily.
    Contains 1 nudix hydrolase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCP2 antibody
    • DCP2 decapping enzyme homolog (S. cerevisiae) antibody
    • DCP2, S. cerevisiae, homolog of antibody
    • DCP2_HUMAN antibody
    • decapping enzyme 2, S. cerevisiae, homolog of antibody
    • decapping enzyme homolog (S. cerevisiae) antibody
    • FLJ33245 antibody
    • hDpc antibody
    • m7GpppN-mRNA hydrolase antibody
    • mRNA decapping enzyme 2 antibody
    • mRNA-decapping enzyme 2 antibody
    • Nucleoside diphosphate-linked moiety X motif 20 antibody
    • nudix (nucleoside diphosphate linked moiety X)-type motif 20 antibody
    • Nudix motif 20 antibody
    • NUDT20 antibody
    see all

Images

  • Anti-Dcp2/TDT antibody (ab127185) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • ab127185, at 1/500 dilution, staining Dcp2/TDT in paraffin-embedded HeLa xenograft tissue by Immunohistochemistry.

References

ab127185 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab127185.
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