Key features and details
- Rabbit polyclonal to Dcp2/TDT
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dcp2/TDT antibody
See all Dcp2/TDT primary antibodies
DescriptionRabbit polyclonal to Dcp2/TDT
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human Dcp2/TDT aa 370-420. The exact sequence is proprietary. NP_689837.2
Database link: Q8IU60
- WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate.
This product was previously labelled as Dcp2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245661 was affinity purified using an epitope specific to Dcp2/TDT immobilized on solid support.
Our Abpromise guarantee covers the use of ab245661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-15 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 48 kDa.|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
Sequence similaritiesBelongs to the Nudix hydrolase family. DCP2 subfamily.
Contains 1 nudix hydrolase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
- Information by UniProt
- DCP2 antibody
- DCP2 decapping enzyme homolog (S. cerevisiae) antibody
- DCP2, S. cerevisiae, homolog of antibody
All lanes : Anti-Dcp2/TDT antibody (ab245661) at 0.4 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 5 µg
Predicted band size: 48 kDa
Exposure time: 3 minutes
Dcp2/TDT was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245661 used for IP at 10 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245661 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245661 has not yet been referenced specifically in any publications.