Product nameAnti-Dcp2/TDT antibody
See all Dcp2/TDT primary antibodies
DescriptionRabbit polyclonal to Dcp2/TDT
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, EMSA, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Dcp2/TDT aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293. This antibody gave a positive signal in the following Mouse whole cell lysate: NIH 3T3 (data not shown).
This product was previously labelled as Dcp2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48.4 kDa).|
|IHC-P||1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|EMSA||Use at an assay dependent concentration. PubMed: 20691152|
|IP||Use at an assay dependent concentration.|
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
Sequence similaritiesBelongs to the Nudix hydrolase family. DCP2 subfamily.
Contains 1 nudix hydrolase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
- Information by UniProt
- DCP2 antibody
- DCP2 decapping enzyme homolog (S. cerevisiae) antibody
- DCP2, S. cerevisiae, homolog of antibody
All lanes : Anti-Dcp2/TDT antibody (ab28658) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Dcp2/TDT peptide (ab30458) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Dcp2/TDT peptide (ab30458) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human Dcp2/TDT peptide (ab30458) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 48.4 kDa
Observed band size: 52,58 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Dcp2/TDT contains a number of potential phosphorylation sites (Swiss Prot data) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab28658 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28658, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas
ab28658 stainning Dcp2/TDT in paraffin embedded human kidney. Kidney tissue sections (4 µm) were incubated with ab28658 (1/35 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab28658 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Dcp2/TDT was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Dcp2/TDT and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab28658.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52,58kDa: Dcp2/TDT.
This product has been referenced in:
- Seto E et al. The Assembly of EDC4 and Dcp1a into Processing Bodies Is Critical for the Translational Regulation of IL-6. PLoS One 10:e0123223 (2015). WB . Read more (PubMed: 25970328) »
- Wang X et al. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature 505:117-20 (2014). WB . Read more (PubMed: 24284625) »