Anti-Dcp2/TDT antibody - C-terminal (ab189966)
Key features and details
- Rabbit polyclonal to Dcp2/TDT - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dcp2/TDT antibody - C-terminal
See all Dcp2/TDT primary antibodies -
Description
Rabbit polyclonal to Dcp2/TDT - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Dcp2/TDT (C terminal). The exact sequence is proprietary. The peptide corresponds to 18 amino acids.
Database link: Q8IU60 -
Positive control
- Small intestine and testis tissue. 293 cell lysate
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General notes
This product was previously labelled as Dcp2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa. | |
| IHC-P | Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. -
Sequence similarities
Belongs to the Nudix hydrolase family. DCP2 subfamily.
Contains 1 nudix hydrolase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear. - Information by UniProt
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Database links
- Entrez Gene: 167227 Human
- Omim: 609844 Human
- SwissProt: Q8IU60 Human
- Unigene: 443875 Human
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Alternative names
- DCP2 antibody
- DCP2 decapping enzyme homolog (S. cerevisiae) antibody
- DCP2, S. cerevisiae, homolog of antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp2/TDT antibody - C-terminal (ab189966)
Immunohistochemistry of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Dcp2/TDT with ab189966 at 10 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp2/TDT antibody - C-terminal (ab189966)Immunohistochemistry of Human testis tissue labeling Dcp2/TDT with ab189966 at 20 µg/ml. Detection by immunofluorescence.
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Western blot - Anti-Dcp2/TDT antibody - C-terminal (ab189966)All lanes : Anti-Dcp2/TDT antibody - C-terminal (ab189966) at 1 µg/ml
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate with Blocking peptide
Predicted band size: 48 kDa
Protocols
Datasheets and documents
References (0)
ab189966 has not yet been referenced specifically in any publications.
