Anti-Dcp2/TDT antibody - C-terminal (ab189966)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Dcp2/TDT antibody - C-terminal
See all Dcp2/TDT primary antibodies -
Description
Rabbit polyclonal to Dcp2/TDT - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Dcp2/TDT (C terminal). The exact sequence is proprietary. The peptide corresponds to 18 amino acids.
Database link: Q8IU60 -
Positive control
- Small intestine and testis tissue. 293 cell lysate
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General notes
This product was previously labelled as Dcp2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa. | |
| IHC-P | Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. -
Sequence similarities
Belongs to the Nudix hydrolase family. DCP2 subfamily.
Contains 1 nudix hydrolase domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear. - Information by UniProt
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Database links
- Entrez Gene: 167227 Human
- Omim: 609844 Human
- SwissProt: Q8IU60 Human
- Unigene: 443875 Human
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Alternative names
- DCP2 antibody
- DCP2 decapping enzyme homolog (S. cerevisiae) antibody
- DCP2, S. cerevisiae, homolog of antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp2/TDT antibody - C-terminal (ab189966)Immunohistochemistry of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Dcp2/TDT with ab189966 at 10 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp2/TDT antibody - C-terminal (ab189966)Immunohistochemistry of Human testis tissue labeling Dcp2/TDT with ab189966 at 20 µg/ml. Detection by immunofluorescence.
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Western blot - Anti-Dcp2/TDT antibody - C-terminal (ab189966)All lanes : Anti-Dcp2/TDT antibody - C-terminal (ab189966) at 1 µg/ml
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate with Blocking peptide
Predicted band size: 48 kDa
Datasheets and documents
References
ab189966 has not yet been referenced specifically in any publications.
