Overview

  • Product name

    Anti-DcR1 antibody [EPR6162]
    See all DcR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6162] to DcR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DcR1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human spleen, JAR, Human tonsil, Jurkat, A549 and Human fetal heart lysates; A549 cells.
  • General notes

    This antibody was developed as part of a collaboration with the lab of Shi-Yong Sun at Emory University.

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27, 75, 80 kDa (predicted molecular weight: 65 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance

      DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
    • Cellular localization

      Cell Membrane and Cytoplasmic
    • Database links

    • Alternative names

      • Antagonist decoy receptor for TRAIL/Apo 2L antibody
      • CD263 antibody
      • Cytotoxic TRAIL receptor 3 antibody
      • DCR1 antibody
      • DCR1 TNFR antibody
      • Decoy Receptor 1 antibody
      • Decoy TRAIL receptor without death domain antibody
      • LIT antibody
      • Lymphocyte inhibitor of TRAIL antibody
      • MGC149501 antibody
      • MGC149502 antibody
      • TNF related apoptosis inducing ligand receptor 3 antibody
      • TNF-re ated apoptosis inducing ligand receptor 3 antibody
      • TNFRSF10C antibody
      • TRAIL R3 antibody
      • TRAIL receptor 3 antibody
      • TRAILR3 antibody
      • TRID antibody
      • Tumor necrosis factor receptor superfamily member 10C antibody
      • Tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain antibody
      see all

    Images

    • All lanes : Anti-DcR1 antibody [EPR6162] (ab133658) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Human tonsil lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : A549 cell lysate
      Lane 6 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunofluorescent analysis of A549 cells labelling DcR1 with ab133658 at 1/100 dilution.

    References

    ab133658 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133658.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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