RecombinantRabMAb

Recombinant Anti-DcR2 antibody [EPR3588(2)] (ab108421)

Submit a review Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
  • Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
  • Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
  • Anti-DcR2 antibody [EPR3588(2)] (ab108421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3588(2)] to DcR2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-DcR2 antibody [EPR3588(2)]
    See all DcR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3588(2)] to DcR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney, Fetal liver, Fetal kidney and Human prostate lysates. IHC-P: human thyroid carcinoma, kidney and tonsil tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500.

See IHC antigen retrieval protocols
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500.

See IHC antigen retrieval protocols

Target

  • Function

    Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.

  • Tissue specificity

    Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.

  • Sequence similarities

    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 264 antibody
      • CD264 antibody
      • DcR 2 antibody
      • DcR2 antibody
      • Decoy receptor 2 antibody
      • Decoy with truncated death domain antibody
      • OTTHUMP00000123528 antibody
      • TNF receptor related receptor for TRAIL antibody
      • TNF related apoptosis inducing ligand receptor 4 antibody
      • TNF-related apoptosis-inducing ligand receptor 4 antibody
      • TNFRSF 10D antibody
      • TNFRSF10D antibody
      • TR10D_HUMAN antibody
      • TRAIL R4 antibody
      • TRAIL receptor 4 antibody
      • TRAIL receptor with a truncated death domain antibody
      • TRAIL-R4 antibody
      • TRAILR4 antibody
      • TRUNDD antibody
      • Tumor necrosis factor receptor superfamily member 10D antibody
      • Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling DcR2 with purified ab108421 at 1:8000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Purified) + Human kidney lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

      Predicted band size: 42 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in PMID: 12865931

    • Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      All lanes : Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Unpurified)

      Lane 1 : Fetal liver lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Human prostate lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)

      Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)

      Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-DcR2 antibody [EPR3588(2)] (ab108421)
      Anti-DcR2 antibody [EPR3588(2)] (ab108421)

    References (1)

    Publishing research using ab108421? Please let us know so that we can cite the reference in this datasheet.

    ab108421 has been referenced in 1 publication.

    • Bird TG  et al. TGFß inhibition restores a regenerative response in acute liver injury by suppressing paracrine senescence. Sci Transl Med 10:N/A (2018). PubMed: 30111642

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Kreuzreaktivität dieser Antikörper?

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    Answer

    Vielen Dank für Ihren Anruf.
    Ich habe nachgefragt ob und wie die Spezifität der folgenden Antikörper getestet wurde:
    ab108421:
    Dieser Antikörper wurde nicht experimentell auf die Kreuzreaktion mit anderen Trail Rezeptoren getestet. Ich kann allerding bestätigen, dass das Immunogen keine signifikante Homologie mit DcR1 und DcR3 aufweist. Wir können dies allerdings nicht garantieren.
    ab2019:
    Dieser Antikörper wurde ebenfalls nicht experimentell auf die Kreuzreaktion mit anderen Trail Rezeptoren getestet. Ich kann allerding bestätigen, dass nach einer BLAST-Suche das Immunogen keine signifikante Homologie mit anderen Trail Rezeptoren aufweist. Wir können dies allerdings nicht garantieren.
    ab14738:
    Die Kreuzreaktivität dieses Antikörpers wurde mittels TRAIL-R2 transfizierten Zellen ausgeschlossen. Es wurden außerdem Zelllinien, die TRAIL-R3 and TRAIL-R4 exprimieren verwendet und so eine Kreuzreaktion ausgeschlossen.
    Ich hoffe, diese Information ist hilfreich. Bitte zögern Sie nicht sich nochmals zu melden, falls Sie weitere Fragen haben.

    Read More

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