Recombinant Anti-DcR2 antibody [EPR3588(2)] (ab108421)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-5-anti-dcr2-antibody-epr3588-2-immunohistochemistry-thyroid-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3588(2)] to DcR2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DcR2 antibody [EPR3588(2)]
See all DcR2 primary antibodies -
Description
Rabbit monoclonal [EPR3588(2)] to DcR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney, Fetal liver, Fetal kidney and Human prostate lysates. IHC-P: human thyroid carcinoma, kidney and tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3588(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
|
| IHC-P |
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
|
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
Target
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Function
Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. -
Tissue specificity
Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8793 Human
- Omim: 603614 Human
- SwissProt: Q9UBN6 Human
- Unigene: 213467 Human
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Alternative names
- CD 264 antibody
- CD264 antibody
- DcR 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling DcR2 with purified ab108421 at 1:8000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-354907-anti-dcr2-antibody-epr3588-2-westernblot-kidney-human.jpg)
Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Purified) + Human kidney lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in PMID: 12865931
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![Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-121140-anti-dcr2-antibody-epr35882-western-blot.jpg)
Western blot - Anti-DcR2 antibody [EPR3588(2)] (ab108421)All lanes : Anti-DcR2 antibody [EPR3588(2)] (ab108421) at 1/1000 dilution (Unpurified)
Lane 1 : Fetal liver lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Human prostate lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-121139-anti-dcr2-antibody-epr35882-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-121138-anti-dcr2-antibody-epr35882-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DcR2 antibody [EPR3588(2)] (ab108421)Unpurified ab108421, at 1/250 dilution, staining DcR2 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-DcR2 antibody [EPR3588(2)] (ab108421)](https://www.abcam.com/ps/products/108/ab108421/Images/ab108421-6-benefits-of-recombinant-antibodies.png)
Anti-DcR2 antibody [EPR3588(2)] (ab108421)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108421 has been referenced in 1 publication.
- Bird TG et al. TGFß inhibition restores a regenerative response in acute liver injury by suppressing paracrine senescence. Sci Transl Med 10:N/A (2018). PubMed: 30111642