Anti-DcR3 antibody [3C5H10] (ab233816)
Key features and details
- Mouse monoclonal [3C5H10] to DcR3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DcR3 antibody [3C5H10]
See all DcR3 primary antibodies -
Description
Mouse monoclonal [3C5H10] to DcR3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DcR3 aa 1 to the C-terminus. Expressed in E.coli.
Database link: O95407 -
Positive control
- WB: Human DcR3 (AA: 30-300) recombinant protein; DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3C5H10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis. -
Tissue specificity
Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8771 Human
- Omim: 603361 Human
- SwissProt: O95407 Human
- Unigene: 434878 Human
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Alternative names
- DcR3 antibody
- Decoy receptor 3 antibody
- Decoy receptor for Fas ligand antibody
see all
Images
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Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution + Human DcR3 (AA: 30-300) recombinant protein.
Expected MW is 55.7 kDa.
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All lanes : Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate. -
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling DcR3 using ab233816 at 1/200 dilution (green) and the negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233816 has not yet been referenced specifically in any publications.