Anti-DcR3 antibody [3C5H10] (ab233816)

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Overview

  • Product name

    Anti-DcR3 antibody [3C5H10]
    See all DcR3 primary antibodies

  • Description

    Mouse monoclonal [3C5H10] to DcR3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human DcR3 aa 30-300. Expressed in E.coli.
    Sequence:

    VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYT QFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEH ASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGL ALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRL QRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQA LRVARMPGLERSVRERFLPVH


    Database link: O95407

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human DcR3 (AA: 30-300) recombinant protein; DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab233816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis.

  • Tissue specificity

    Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

  • Sequence similarities

    Contains 4 TNFR-Cys repeats.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • DcR3 antibody
      • Decoy receptor 3 antibody
      • Decoy receptor for Fas ligand antibody
      • DJ583P15.1.1 antibody
      • M68 antibody
      • M68E antibody
      • OTTHUMP00000031583 antibody
      • TNF6B_HUMAN antibody
      • TNFRSF6B antibody
      • TR6 antibody
      • Tumor necrosis factor receptor superfamily member 6B antibody
      • Tumor necrosis factor receptor superfamily member 6b decoy antibody
      • UNQ186/PRO212 antibody
      see all

    Images

    • Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution + Human DcR3 (AA: 30-300) recombinant protein.


      Expected MW is 55.7 kDa.

    • Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      All lanes : Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
      Lane 2 : DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate.
    • Flow Cytometry - Anti-DcR3 antibody [3C5H10] (ab233816)
      Flow Cytometry - Anti-DcR3 antibody [3C5H10] (ab233816)

      Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling DcR3 using ab233816 at 1/200 dilution (green) and the negative control (red).

    References

    ab233816 has not yet been referenced specifically in any publications.

    Publishing research using ab233816? Please let us know so that we can cite the reference in this datasheet.

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