Key features and details
- Mouse monoclonal [3C5H10] to DcR3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-DcR3 antibody [3C5H10]
See all DcR3 primary antibodies
DescriptionMouse monoclonal [3C5H10] to DcR3
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DcR3 aa 30-300. Expressed in E.coli.
VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYT QFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEH ASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGL ALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRL QRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQA LRVARMPGLERSVRERFLPVH
Database link: O95407
- WB: Human DcR3 (AA: 30-300) recombinant protein; DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|Flow Cyt||1/200 - 1/400.|
FunctionDecoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis.
Tissue specificityDetected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
Sequence similaritiesContains 4 TNFR-Cys repeats.
- Information by UniProt
- DcR3 antibody
- Decoy receptor 3 antibody
- Decoy receptor for Fas ligand antibody
Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution + Human DcR3 (AA: 30-300) recombinant protein.
Expected MW is 55.7 kDa.
All lanes : Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate.
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling DcR3 using ab233816 at 1/200 dilution (green) and the negative control (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233816 has not yet been referenced specifically in any publications.