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  1. Link

    dcr3-antibody-3c5h10-ab233816.pdf

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Cell Biology Apoptosis Receptors Decoy Receptors
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Anti-DcR3 antibody [3C5H10] (ab233816)

  • Datasheet
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Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
  • Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
  • Flow Cytometry - Anti-DcR3 antibody [3C5H10] (ab233816)

Key features and details

  • Mouse monoclonal [3C5H10] to DcR3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human DcR3 protein (ab157279)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-DcR3 antibody [3C5H10]
    See all DcR3 primary antibodies
  • Description

    Mouse monoclonal [3C5H10] to DcR3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DcR3 aa 30-300. Expressed in E.coli.
    Sequence:

    VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYT QFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEH ASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGL ALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRL QRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQA LRVARMPGLERSVRERFLPVH


    Database link: O95407
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human DcR3 (AA: 30-300) recombinant protein; DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate. Flow: HL-60 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3C5H10
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Decoy Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Necrosis Factors
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Decoy Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human DcR3 protein (ab157279)
  • Related Products

    • Recombinant Human DcR3 protein (denatured) (ab176068)

Applications

Our Abpromise guarantee covers the use of ab233816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis.
  • Tissue specificity

    Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O95407 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8771 Human
    • Omim: 603361 Human
    • SwissProt: O95407 Human
    • Unigene: 434878 Human
    • Alternative names

      • DcR3 antibody
      • Decoy receptor 3 antibody
      • Decoy receptor for Fas ligand antibody
      • DJ583P15.1.1 antibody
      • M68 antibody
      • M68E antibody
      • OTTHUMP00000031583 antibody
      • TNF6B_HUMAN antibody
      • TNFRSF6B antibody
      • TR6 antibody
      • Tumor necrosis factor receptor superfamily member 6B antibody
      • Tumor necrosis factor receptor superfamily member 6b decoy antibody
      • UNQ186/PRO212 antibody
      see all

    Images

    • Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution + Human DcR3 (AA: 30-300) recombinant protein.


      Expected MW is 55.7 kDa.

    • Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      Western blot - Anti-DcR3 antibody [3C5H10] (ab233816)
      All lanes : Anti-DcR3 antibody [3C5H10] (ab233816) at 1/500 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
      Lane 2 : DcR3 (AA: 30-300)-hIgGFc transfected HEK-293 cell lysate.
    • Flow Cytometry - Anti-DcR3 antibody [3C5H10] (ab233816)
      Flow Cytometry - Anti-DcR3 antibody [3C5H10] (ab233816)

      Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling DcR3 using ab233816 at 1/200 dilution (green) and the negative control (red).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab233816? Please let us know so that we can cite the reference in this datasheet.

    ab233816 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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