Recombinant Anti-DCTN1 antibody [EPR26464-28] (ab302629)
![Western blot - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-489472-anti-dctn1p150glued-antibody-epr2646428-western-blot-hela.jpg "Western blot - Anti-DCTN1 antibody [EPR26464-28] (AB302629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26464-28] to DCTN1/p150-glued
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-DCTN1 antibody [EPR26464-28]
See all DCTN1/p150-glued primary antibodies -
Description
Rabbit monoclonal [EPR26464-28] to DCTN1/p150-glued -
Host species
Rabbit -
Specificity
IHC application not suitable for human and rat species.
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Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IPmore details -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa transfected with siRNA specifically targeting DCTN1 and a scrambled siRNA control, Neuro-2a, and NIH/3T3 whole cell lysates, mouse brain and cerecbellum tissue lysates. IHC-P: Mouse cerebrum, spinal cord, and dorsal root ganglion FFPE tissue sections. IHC-Fr: Mouse spinal cord fresh frozen tissue section. ICC/IF: HeLa and Neuro-2a cell lines. Flow Cyt (Intra): HeLa and Neuro-2a cell lines. IP: HeLa and Neuro-2a whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26464-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa).
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
1/100.
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| ICC/IF |
1/50.
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| Flow Cyt (Intra) |
1/50.
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| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IHC-Fr
1/100. |
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ICC/IF
1/50. |
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Flow Cyt (Intra)
1/50. |
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IP
1/30. |
Target
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Function
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. -
Tissue specificity
Brain. -
Involvement in disease
Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. -
Sequence similarities
Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain. -
Post-translational
modificationsUbiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 1639 Human
- Entrez Gene: 13191 Mouse
- Omim: 601143 Human
- SwissProt: Q14203 Human
- SwissProt: O08788 Mouse
- Unigene: 516111 Human
- Unigene: 6919 Mouse
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Alternative names
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
see all
Images
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Western blot - Anti-DCTN1 antibody [EPR26464-28] (AB302629)All lanes : Anti-DCTN1 antibody [EPR26464-28] (ab302629) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate
Lane 2 : Hela transfected with siRNA specifically targeting DCTN1, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 114 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-489471-anti-dctn1p150glued-antibody-epr2646428-western-blot-hela-2.jpg)
Western blot - Anti-DCTN1 antibody [EPR26464-28] (AB302629)All lanes : Anti-DCTN1 antibody [EPR26464-28] (ab302629) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse cerecbellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-8-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cerebrum (PMID:17122035). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-9-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-dorsal-root-ganglia.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of paraffin-embedded mouse dorsal root ga tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse dorsal root ganglion (PMID:23874158). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-10-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-spinal-cord.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse spinal cord (PMID:23408943, 17122035 ). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-11-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse liver (PMID:1836789). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Frozen sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-6-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-frozen-mouse-spinal-cord.jpg)
Immunohistochemistry (Frozen sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spinal cord (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse spinal cord is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Immunohistochemistry (Frozen sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-7-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-frozen-mouse-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on mouse liver (PMID:1836789) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-4-anti-dctn1p150glued-antibody-epr2646428-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling DCTN1/p150-glued with ab302629 at 1/50 (10.78 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/ml dilution) (Green). Confocal image showing mainly cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-5-anti-dctn1p150glued-antibody-epr2646428-immunocytochemistry-neuro2a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1 antibody [EPR26464-28] (AB302629)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DCTN1/p150-glued with ab302629 at 1/50 (10.78 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/ml) dilution.
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![Flow Cytometry (Intracellular) - Anti-DCTN1 antibody [EPR26464-28] (ab302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-2-anti-dctn1p150glued-antibody-epr2646428-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-DCTN1 antibody [EPR26464-28] (ab302629)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling DCTN1/p150-glued with ab302629 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-DCTN1 antibody [EPR26464-28] (ab302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-3-anti-dctn1p150glued-antibody-epr2646428-flow-cytometry-neuro2a.jpg)
Flow Cytometry (Intracellular) - Anti-DCTN1 antibody [EPR26464-28] (ab302629)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DCTN1/p150-glued with ab302629 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-12-anti-dctn1p150glued-antibody-epr2646428-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-DCTN1 antibody [EPR26464-28] (AB302629)DCTN1/p150-glued was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg
Lane 2: ab302629 IP in HeLa whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab302629 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Immunoprecipitation - Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-13-anti-dctn1p150glued-antibody-epr2646428-immunoprecipitation-neuro2a.jpg)
Immunoprecipitation - Anti-DCTN1 antibody [EPR26464-28] (AB302629)DCTN1/p150-glued was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg
Lane 2: ab302629 IP in Neuro-2a whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab302629 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Anti-DCTN1 antibody [EPR26464-28] (AB302629)](https://www.abcam.com/ps/products/302/ab302629/Images/AB302629-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-DCTN1 antibody [EPR26464-28] (AB302629)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302629 has not yet been referenced specifically in any publications.