• Product name
    Anti-DCTN1/p150-glued antibody
    See all DCTN1/p150-glued primary antibodies
  • Description
    Goat polyclonal to DCTN1/p150-glued
  • Host species
  • Specificity
    This antibody is expected to recognise both human isoforms.
  • Tested applications
    Suitable for: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1266-1278 (C terminal).


    (Peptide available as ab23214)

  • Positive control
    • A549 and Human Testis lysates.
  • General notes

    Previously labelled as DCTN1. 



Our Abpromise guarantee covers the use of ab11806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution. PubMed: 17932487
IHC-Fr Use at an assay dependent dilution. PubMed: 17932487
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.Can be blocked with Human DCTN1/p150-glued peptide (ab23214). Approx 150kDa band observed in A549 and Human Testis lysates


  • Function
    Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
  • Tissue specificity
  • Involvement in disease
    Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
  • Sequence similarities
    Belongs to the dynactin 150 kDa subunit family.
    Contains 1 CAP-Gly domain.
  • Post-translational
    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 150 kDa dynein associated polypeptide antibody
    • 150 kDa dynein-associated polypeptide antibody
    • DAP 150 antibody
    • DAP-150 antibody
    • DAP150 antibody
    • DCTN 1 antibody
    • DCTN1 antibody
    • DCTN1_HUMAN antibody
    • DP 150 antibody
    • DP-150 antibody
    • DP150 antibody
    • Dynactin 1 (p150 Glued (Drosophila) homolog) antibody
    • Dynactin 1 (p150 glued homolog Drosophila) antibody
    • Dynactin 1 antibody
    • Dynactin subunit 1 antibody
    • Dynactin1 antibody
    • HMN7B antibody
    • p135 antibody
    • p150 Glued (Drosophila) homolog antibody
    • p150 glued antibody
    • p150 glued homolog antibody
    • p150(GLUED) DROSOPHILA HOMOLOG OF antibody
    • p150-glued antibody
    • p150glued antibody
    see all


  • Anti-DCTN1/p150-glued antibody (ab11806) at 1 µg/ml + Human testis tissue lysate at 35 µg

    Predicted band size: 150 kDa

    Ab11806 staining (1µg/ml) of Human testis lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab11806 at 2.5ug/ml staining DCTN1/p150-glued in human cerebellum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab11806 staining DCTN1/p150-glued in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.5% Triton X and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of murine colon tissue, staining DCTN1/p150-glued with ab11806.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Sun L  et al. TAR DNA Binding Protein-43 Loss of Function Induced by Phosphorylation at S409/410 Blocks Autophagic Flux and Participates in Secondary Brain Injury After Intracerebral Hemorrhage. Front Cell Neurosci 12:79 (2018). Read more (PubMed: 29623031) »
  • Villarin JM  et al. Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands. Nat Commun 7:13865 (2016). Read more (PubMed: 28000671) »
See all 8 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with zebrafish has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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