Product nameAnti-DCTN1/p150-glued antibody
See all DCTN1/p150-glued primary antibodies
DescriptionGoat polyclonal to DCTN1/p150-glued
SpecificityThis antibody is expected to recognise both human isoforms.
Tested applicationsSuitable for: IHC-P, ICC, ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Drosophila melanogaster
- A549 and Human Testis lysates.
Previously labelled as DCTN1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab11806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17932487|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 17932487|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.
A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates
FunctionRequired for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
Involvement in diseaseDefects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
Sequence similaritiesBelongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain.
modificationsUbiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton.
- Information by UniProt
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
Anti-DCTN1/p150-glued antibody (ab11806) at 1 µg/ml + Human testis tissue lysate at 35 µg
Predicted band size: 150 kDa
Ab11806 staining (1Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
µg/ml) of Human testis lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab11806 at 2.5ug/ml staining DCTN1/p150-glued in human cerebellum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab11806 staining DCTN1/p150-glued in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.5% Triton X and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal (1/2000) was used as the secondary antibody.
Immunohistochemical analysis of murine colon tissue, staining DCTN1/p150-glued with ab11806.
Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.
ab11806 has been referenced in 8 publications.
- Sun L et al. TAR DNA Binding Protein-43 Loss of Function Induced by Phosphorylation at S409/410 Blocks Autophagic Flux and Participates in Secondary Brain Injury After Intracerebral Hemorrhage. Front Cell Neurosci 12:79 (2018). PubMed: 29623031
- Villarin JM et al. Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands. Nat Commun 7:13865 (2016). PubMed: 28000671
- Chanduri M et al. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 473:3031-47 (2016). PubMed: 27474409
- Jovasevic V et al. Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells. J Cell Biol 211:323-37 (2015). PubMed: 26504169
- Roher AE et al. Subjects harboring presenilin familial Alzheimer's disease mutations exhibit diverse white matter biochemistry alterations. Am J Neurodegener Dis 2:187-207 (2013). PubMed: 24093083
- Williams SE et al. Asymmetric cell divisions promote Notch-dependent epidermal differentiation. Nature 470:353-8 (2011). IHC-Fr ; Mouse . PubMed: 21331036
- Wider C et al. Elucidating the genetics and pathology of Perry syndrome. J Neurol Sci 289:149-54 (2010). PubMed: 19732908
- Magnani E et al. Interaction of tau protein with the dynactin complex. EMBO J 26:4546-54 (2007). WB, ICC/IF, IHC-Fr ; Human, Mouse . PubMed: 17932487