Western blot abreview for Anti-DCTN1 / p150-glued antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HELA)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
HELA
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: DONKEY ANTI-GOAT
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 130 kDa Non-specific: 250 kDa

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Submitted Feb 12 2011

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