Western blot abreview for Anti-DCTN1/p150-glued antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: RABBIT ANTI-GOAT
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 150 kDa

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Submitted Feb 22 2011

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