Overview

  • Product name

    Anti-DCTN1/p150-glued antibody
    See all DCTN1/p150-glued primary antibodies
  • Description

    Rabbit polyclonal to DCTN1/p150-glued
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1216-1248.
    Database link: NP_004073

  • Positive control

    • Jurkat whole cell lysate (ab7899) for WB. HeLa cells for IF. OV CA for IHC-P.
  • General notes

    Previously labelled as DCTN1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab96004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 142 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
  • Tissue specificity

    Brain.
  • Involvement in disease

    Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
  • Sequence similarities

    Belongs to the dynactin 150 kDa subunit family.
    Contains 1 CAP-Gly domain.
  • Post-translational
    modifications

    Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 kDa dynein associated polypeptide antibody
    • 150 kDa dynein-associated polypeptide antibody
    • DAP 150 antibody
    • DAP-150 antibody
    • DAP150 antibody
    • DCTN 1 antibody
    • DCTN1 antibody
    • DCTN1_HUMAN antibody
    • DP 150 antibody
    • DP-150 antibody
    • DP150 antibody
    • Dynactin 1 (p150 Glued (Drosophila) homolog) antibody
    • Dynactin 1 (p150 glued homolog Drosophila) antibody
    • Dynactin 1 antibody
    • Dynactin subunit 1 antibody
    • Dynactin1 antibody
    • HMN7B antibody
    • p135 antibody
    • p150 Glued (Drosophila) homolog antibody
    • p150 glued antibody
    • p150 glued homolog antibody
    • p150(GLUED) DROSOPHILA HOMOLOG OF antibody
    • p150-glued antibody
    • p150glued antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : NCI-H929 cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse lung

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

    Lane 1 : U87-MG cell lysates
    Lane 2 : SK-N-SH cell lysates
    Lane 3 : IMR32 cell lysates
    Lane 4 : SK-N-AS cell lysates

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

  • Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 142 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

References

ab96004 has not yet been referenced specifically in any publications.

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