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    dctn3-antibody-epr5097-ab124674.pdf

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RecombinantRabMAb

Recombinant Anti-DCTN3 antibody [EPR5097] (ab124674)

  • Datasheet
  • SDS
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Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] (ab124674)
  • Anti-DCTN3 antibody [EPR5097] (ab124674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5097] to DCTN3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-CSNK2A2 antibody (ab10474)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-DCTN3 antibody [EPR5097]
    See all DCTN3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5097] to DCTN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DCTN3 aa 100-200. The exact sequence is proprietary.
    Database link: O75935

  • Positive control

    • 293T, HeLa, BxPC 3, SH-SY5Y and mouse and rat muscle lysates; Human breast tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.58 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5097
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein

Associated products

  • Alternative Versions

    • Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/2000 - 1/10000.
WB (4)
1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
ICC/IF
1/2000 - 1/10000.
WB
1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Together with dynein may be involved in spindle assembly and cytokinesis.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
  • Sequence similarities

    Belongs to the dynactin subunit 3 family.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
  • Target information above from: UniProt accession O75935 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11258 Human
    • Entrez Gene: 53598 Mouse
    • Entrez Gene: 362504 Rat
    • Omim: 607387 Human
    • SwissProt: O75935 Human
    • SwissProt: Q9Z0Y1 Mouse
    • Unigene: 511768 Human
    • Unigene: 29025 Mouse
    • Alternative names

      • DCNT22 antibody
      • DCTN 22 antibody
      • DCTN22 antibody
      • Dctn3 antibody
      • DCTN3_HUMAN antibody
      • dynactin 3 (p22) antibody
      • dynactin 3 antibody
      • dynactin 3 isoform 1 antibody
      • Dynactin complex subunit 22 kDa subunit antibody
      • dynactin light chain antibody
      • Dynactin subunit 3 antibody
      • p22 antibody
      see all

    Images

    • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

      Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

      Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)

      Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.

    • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
      All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysates
      Lane 2 : Mouse muscle lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : BxPC 3 cell lysates
      Lane 5 : SH-SY5Y cell lysates
      Lane 6 : Rat muscle lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-3 & 5-6 : Goat anti-Rabbit HRP at 1/2000 dilution


      Predicted band size: 21 kDa

    • OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] (ab124674)
      OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] (ab124674)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-DCTN3 antibody [EPR5097] (ab124674)
      Anti-DCTN3 antibody [EPR5097] (ab124674)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124674? Please let us know so that we can cite the reference in this datasheet.

    ab124674 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 25 2017

    Western blot abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 25 2017

    Western blot abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 25 2017

    Western blot abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 25 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DCTN3 antibody [EPR5097]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 20 2017

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