- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-DCTN3 antibody [EPR5097]
See all DCTN3 primary antibodies -
DescriptionRabbit monoclonal [EPR5097] to DCTN3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human DCTN3 aa 100-200. The exact sequence is proprietary.
Database link: O75935 -
Positive control
- 293T, HeLa, BxPC 3, SH-SY5Y and mouse and rat muscle lysates; Human breast tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 5.58 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR5097
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab124674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/2000 - 1/10000. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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FunctionTogether with dynein may be involved in spindle assembly and cytokinesis.
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Tissue specificityUbiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
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Sequence similaritiesBelongs to the dynactin subunit 3 family.
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Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
- Information by UniProt
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Database links
- Entrez Gene: 11258 Human
- Entrez Gene: 53598 Mouse
- Entrez Gene: 362504 Rat
- Omim: 607387 Human
- SwissProt: O75935 Human
- SwissProt: Q9Z0Y1 Mouse
- Unigene: 511768 Human
- Unigene: 29025 Mouse
see all -
Alternative names
- DCNT22 antibody
- DCTN 22 antibody
- DCTN22 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/DCTN3-Primary-antibodies-ab124674-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/ab124674-240076-124674IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/ab124674-240077-124674-ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.
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![Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/ab124674-240073-124674-WB-1.jpg)
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/ab124674-240075-124674-WB-3.jpg)
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/ab124674-240074-124674-WB-2.jpg)
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/DCTN3-Primary-antibodies-ab124674-1.jpg)
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysates
Lane 2 : Mouse muscle lysates
Lane 3 : HeLa cell lysates
Lane 4 : BxPC 3 cell lysates
Lane 5 : SH-SY5Y cell lysates
Lane 6 : Rat muscle lysates
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 & 5-6 : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa -
![Other - Anti-DCTN3 antibody [EPR5097] (ab124674)](https://www.abcam.com/ps/products/124/ab124674/Images/DCTN3-Primary-antibodies-ab124674-3.jpg)
Other - Anti-DCTN3 antibody [EPR5097] (ab124674)
Protocols
References
ab124674 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"