Name: Anti-DCTN3 antibody [EPR5097]
Brand: RabMAb
SKU: ab124674
Rating: 4 (6 reviews)

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Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5097] to DCTN3
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

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Primary

Secondary

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Primary - Rabbit Anti-DCTN3 antibody [EPR5097] (ab124674)
ICC/IF, WB, IHC-P

Secondary - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr

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Product name

Anti-DCTN3 antibody [EPR5097]
See all DCTN3 primary antibodies
Description

Rabbit monoclonal [EPR5097] to DCTN3
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human DCTN3 aa 100-200. The exact sequence is proprietary.
Database link: O75935
Positive control
- 293T, HeLa, BxPC 3, SH-SY5Y and mouse and rat muscle lysates; Human breast tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.58 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5097
Isotype

IgG
Research areas

Alternative Versions
- Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab124674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/2000 - 1/10000.
WB		1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P		1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Function

Together with dynein may be involved in spindle assembly and cytokinesis.
Tissue specificity

Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
Sequence similarities

Belongs to the dynactin subunit 3 family.
Cellular localization

Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
Target information above from: UniProt accession O75935 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 11258 Human
- Entrez Gene: 53598 Mouse
- Entrez Gene: 362504 Rat
- Omim: 607387 Human
- SwissProt: O75935 Human
- SwissProt: Q9Z0Y1 Mouse
- Unigene: 511768 Human
- Unigene: 29025 Mouse
- Unigene: 2038 Rat
see all
Alternative names
- DCNT22 antibody
- DCTN 22 antibody
- DCTN22 antibody
- Dctn3 antibody
- DCTN3_HUMAN antibody
- dynactin 3 (p22) antibody
- dynactin 3 antibody
- dynactin 3 isoform 1 antibody
- Dynactin complex subunit 22 kDa subunit antibody
- dynactin light chain antibody
- Dynactin subunit 3 antibody
- p22 antibody
see all

Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)

All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)

Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)

Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)

Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)

Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)

All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysates
Lane 2 : Mouse muscle lysates
Lane 3 : HeLa cell lysates
Lane 4 : BxPC 3 cell lysates
Lane 5 : SH-SY5Y cell lysates
Lane 6 : Rat muscle lysates

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 & 5-6 : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa
Other - Anti-DCTN3 antibody [EPR5097] (ab124674)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab124674? Please let us know so that we can cite the reference in this datasheet.

ab124674 has not yet been referenced specifically in any publications.

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Application

Western blot

Sample

African green monkey Cell lysate - whole cell (Kidney)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Kidney)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2017

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Brain)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2017

Application

Western blot

Sample

Cow Cell lysate - whole cell (Lung)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Lung

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - 0.3% triton x 100

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

African green monkey Cell (Kidney)

Permeabilization

Yes - 0.2% triton x 100

Specification

Kidney

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 20 2017

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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-DCTN3 antibody [EPR5097]

Dissociation constant (K_D)