Recombinant
RabMAb

Recombinant Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

Overview

  • Product name

    Anti-DCTN3 antibody [EPR5097] - BSA and Azide free
    See all DCTN3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5097] to DCTN3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DCTN3 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O75935

  • Positive control

    • IHC-P: Human pancreas tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232440 is a PBS-only buffer format of ab124674. Please refer to ab124674 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Together with dynein may be involved in spindle assembly and cytokinesis.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
  • Sequence similarities

    Belongs to the dynactin subunit 3 family.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCNT22 antibody
    • DCTN 22 antibody
    • DCTN22 antibody
    • Dctn3 antibody
    • DCTN3_HUMAN antibody
    • dynactin 3 (p22) antibody
    • dynactin 3 antibody
    • dynactin 3 isoform 1 antibody
    • Dynactin complex subunit 22 kDa subunit antibody
    • dynactin light chain antibody
    • Dynactin subunit 3 antibody
    • p22 antibody
    see all

Images

  • Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

References

ab232440 has not yet been referenced specifically in any publications.

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