Recombinant Anti-DCTN4 antibody [EPR11223] (ab170107)
![Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/ab170107-170048-anti-dctn4-antibody-epr11223-western-blot.jpg "Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11223] to DCTN4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-DCTN4 antibody [EPR11223]
See all DCTN4 primary antibodies -
Description
Rabbit monoclonal [EPR11223] to DCTN4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human DCTN4.
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Positive control
- Human fetal brain, U87-MG, K562 or HeLa lysate, Human kidney tissue, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11223 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
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ICC/IF
1/100 - 1/250. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton. -
Sequence similarities
Belongs to the dynactin subunit 4 family. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels. - Information by UniProt
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Database links
- Entrez Gene: 51164 Human
- Entrez Gene: 67665 Mouse
- Entrez Gene: 84428 Rat
- SwissProt: Q9UJW0 Human
- SwissProt: Q8CBY8 Mouse
- SwissProt: Q9QUR2 Rat
- Unigene: 675564 Human
- Unigene: 272801 Mouse
see all -
Alternative names
- DCTN 4 antibody
- Dctn4 antibody
- DCTN4_HUMAN antibody
see all
Images
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Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)All lanes : Anti-DCTN4 antibody [EPR11223] (ab170107) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/ab170107-170049-anti-dctn4-antibody-epr11223-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling DCTN4 with ab170107 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/ab170107-170047-anti-dctn4-antibody-epr11223-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)Immunofluorescence analysis of HeLa cells labeling DCTN4 with ab170107 at a 1/100 dilution (red) plus Dapi staining (blue). -
![Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/ab170107-4-benefits-of-recombinant-antibodies.png)
Anti-DCTN4 antibody [EPR11223] (ab170107)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab170107 has not yet been referenced specifically in any publications.
