Recombinant
Anti-DCTN4 antibody [EPR11223] (ab170107)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DCTN4 antibody [EPR11223]
See all DCTN4 primary antibodies -
DescriptionRabbit monoclonal [EPR11223] to DCTN4
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Human DCTN4.
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Positive control
- Human fetal brain, U87-MG, K562 or HeLa lysate, Human kidney tissue, HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11223
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 62 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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FunctionCould have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
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Sequence similaritiesBelongs to the dynactin subunit 4 family.
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Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels.
- Information by UniProt
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Database links
- Entrez Gene: 51164 Human
- Entrez Gene: 67665 Mouse
- Entrez Gene: 84428 Rat
- SwissProt: Q9UJW0 Human
- SwissProt: Q8CBY8 Mouse
- SwissProt: Q9QUR2 Rat
- Unigene: 675564 Human
- Unigene: 272801 Mouse
see all -
Alternative names
- DCTN 4 antibody
- Dctn4 antibody
- DCTN4_HUMAN antibody
see all
Images
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![Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/DCTN4-Primary-antibodies-ab170107-4.jpg)
Western blot - Anti-DCTN4 antibody [EPR11223] (ab170107)All lanes : Anti-DCTN4 antibody [EPR11223] (ab170107) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U87-MG cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/DCTN4-Primary-antibodies-ab170107-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN4 antibody [EPR11223] (ab170107)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling DCTN4 with ab170107 at a 1/50 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)](https://www.abcam.com/ps/products/170/ab170107/Images/DCTN4-Primary-antibodies-ab170107-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN4 antibody [EPR11223] (ab170107)Immunofluorescence analysis of HeLa cells labeling DCTN4 with ab170107 at a 1/100 dilution (red) plus Dapi staining (blue).
Protocols
Datasheets and documents
References
ab170107 has not yet been referenced specifically in any publications.
