Recombinant Anti-DCTN5 antibody [EPR11253] (ab157197)
![Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/products/157/ab157197/Images/ab157197-164367-anti-dctn5-antibody-epr11253-western-blot.jpg "Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11253] to DCTN5
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies -
Description
Rabbit monoclonal [EPR11253] to DCTN5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human DCTN5.
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Positive control
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11253 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| ICC/IF | (1) |
1/250 - 1/500.
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
|
IP
1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 84516 Human
- Entrez Gene: 59288 Mouse
- Entrez Gene: 308961 Rat
- Omim: 612962 Human
- SwissProt: Q9BTE1 Human
- SwissProt: Q9QZB9 Mouse
- Unigene: 435941 Human
- Unigene: 478285 Mouse
see all -
Alternative names
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
see all
Images
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Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/products/157/ab157197/Images/ab157197-164366-anti-dctn5-antibody-epr11253-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/products/157/ab157197/Images/ab157197-164365-anti-dctn5-antibody-epr11253-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution. -
![Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/products/157/ab157197/Images/ab157197-164364-anti-dctn5-antibody-epr11253-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution. -
![Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/products/157/ab157197/Images/ab157197-5-benefits-of-recombinant-antibodies.png)
Anti-DCTN5 antibody [EPR11253] (ab157197)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157197 has not yet been referenced specifically in any publications.