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Rabbit monoclonal [EPR11253] to DCTN5
, IPmore detailsUnsuitable for:
Mouse, Rat, Human
Recombinant fragment corresponding to Human DCTN5.
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
||1/250 - 1/500.
||1/10 - 1/100.
Is unsuitable for Flow Cyt.
Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.
Cytoplasm > cytoskeleton.
Information by UniProt
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)
All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)
Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)
Detection of DCTN5 by Western Blot of Immunprecipitate.
MCF7 cell lysate immunoprecipitated using ab156197
at 1/10 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"