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RecombinantRabMAb

Recombinant Anti-DCTN5 antibody [EPR11253] (ab157197)

  • Datasheet
  • SDS
Reviews (6) Submit a question

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Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)
  • Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)
  • Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)
  • Anti-DCTN5 antibody [EPR11253] (ab157197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11253] to DCTN5
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Recombinant Human DCTN5 protein (ab182817)

View more associated products

Overview

  • Product name

    Anti-DCTN5 antibody [EPR11253]
    See all DCTN5 primary antibodies
  • Description

    Rabbit monoclonal [EPR11253] to DCTN5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human DCTN5.

  • Positive control

    • MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11253
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-DCTN5 antibody [EPR11253] - BSA and Azide free (ab249304)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
  • Recombinant Protein

    • Recombinant Human DCTN5 protein (ab182817)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF (1)
1/250 - 1/500.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities

      Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession Q9BTE1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 84516 Human
      • Entrez Gene: 59288 Mouse
      • Entrez Gene: 308961 Rat
      • Omim: 612962 Human
      • SwissProt: Q9BTE1 Human
      • SwissProt: Q9QZB9 Mouse
      • Unigene: 435941 Human
      • Unigene: 478285 Mouse
      • Unigene: 25812 Rat
      see all
    • Alternative names

      • DCTN 5 antibody
      • DCTN5 antibody
      • DCTN5_HUMAN antibody
      • Dynactin 4 antibody
      • Dynactin 5 (p25) antibody
      • Dynactin subunit 5 antibody
      • Dynactin subunit p25 antibody
      • p25 antibody
      see all

    Images

    • Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)
      All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Human fetal liver cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
    • Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)
      Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.
    • Anti-DCTN5 antibody [EPR11253] (ab157197)
      Anti-DCTN5 antibody [EPR11253] (ab157197)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab157197? Please let us know so that we can cite the reference in this datasheet.

    ab157197 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DCTN5 antibody [EPR11253]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-DCTN5 antibody [EPR11253]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 13 2017

    Western blot abreview for Anti-DCTN5 antibody [EPR11253]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

    Western blot abreview for Anti-DCTN5 antibody [EPR11253]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

    Western blot abreview for Anti-DCTN5 antibody [EPR11253]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

    Western blot abreview for Anti-DCTN5 antibody [EPR11253]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2017

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