Name: Anti-DCTN5 antibody [EPR11253]
Brand: RabMAb
SKU: ab157197
Rating: 5 (6 reviews)

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Primary

Protein

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Primary - Rabbit Anti-DCTN5 antibody [EPR11253] (ab157197)
WB, IHC-P, ICC/IF, IP

Protein - Recombinant Human DCTN5 protein (ab182817)
SDS-PAGE

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Product name

Anti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies
Description

Rabbit monoclonal [EPR11253] to DCTN5
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment corresponding to Human DCTN5.
Positive control
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11253
Isotype

IgG
Research areas

Alternative Versions
- Anti-DCTN5 antibody [EPR11253] - BSA and Azide free (ab249304)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 whole cell lysate (ab29537)
- MCF7 whole cell lysate (ab3871)
Recombinant Protein
- Recombinant Human DCTN5 protein (ab182817)

Our Abpromise guarantee covers the use of ab157197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
IP		1/10 - 1/100.

Application notes

Is unsuitable for Flow Cyt.

Sequence similarities

Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.
Cellular localization

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession Q9BTE1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 84516 Human
- Entrez Gene: 59288 Mouse
- Entrez Gene: 308961 Rat
- Omim: 612962 Human
- SwissProt: Q9BTE1 Human
- SwissProt: Q9QZB9 Mouse
- Unigene: 435941 Human
- Unigene: 478285 Mouse
- Unigene: 25812 Rat
see all
Alternative names
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
- Dynactin 4 antibody
- Dynactin 5 (p25) antibody
- Dynactin subunit 5 antibody
- Dynactin subunit p25 antibody
- p25 antibody
see all

Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)

All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)

Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)

Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

ab157197 has not yet been referenced specifically in any publications.

Publishing research using ab157197? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

African green monkey Cell (Kidney)

Permeabilization

Yes - 0.2% triton x 100

Specification

Kidney

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted May 18 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - 0.3% triton x100

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 13 2017

Application

Western blot

Sample

Cow Cell lysate - whole cell (Lung)

Gel Running Conditions

Non-reduced Non-Denaturing (Native)

Loading amount

30 µg

Specification

Lung

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 05 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Kidney)

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (4-12)

Loading amount

30 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 05 2017

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Brain)

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (4-12)

Loading amount

30 µg

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 05 2017

Application

Western blot

Sample

African green monkey Cell lysate - whole cell (Kidney)

Gel Running Conditions

Non-reduced Non-Denaturing (Native)

Loading amount

30 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 05 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-DCTN5 antibody [EPR11253]

Immunohistochemistry (Frozen sections) abreview for Anti-DCTN5 antibody [EPR11253]

Western blot abreview for Anti-DCTN5 antibody [EPR11253]

Western blot abreview for Anti-DCTN5 antibody [EPR11253]

Western blot abreview for Anti-DCTN5 antibody [EPR11253]

Western blot abreview for Anti-DCTN5 antibody [EPR11253]