Product nameAnti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies
DescriptionRabbit monoclonal [EPR11253] to DCTN5
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human DCTN5.
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 20 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
Sequence similaritiesBelongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.
ab157197 has not yet been referenced specifically in any publications.