- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies -
DescriptionRabbit monoclonal [EPR11253] to DCTN5
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Human DCTN5.
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Positive control
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11253
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 20 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/250 - 1/500. | |
| IP | 1/10 - 1/100. |
Target
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Sequence similaritiesBelongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.
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Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 84516 Human
- Entrez Gene: 59288 Mouse
- Entrez Gene: 308961 Rat
- Omim: 612962 Human
- SwissProt: Q9BTE1 Human
- SwissProt: Q9QZB9 Mouse
- Unigene: 435941 Human
- Unigene: 478285 Mouse
see all -
Alternative names
- DCTN 5 antibody
- DCTN5 antibody
- DCTN5_HUMAN antibody
see all
Images
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![Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/datasheet/images/157/ab157197/DCTN5-Primary-antibodies-ab157197-2.jpg)
Western blot - Anti-DCTN5 antibody [EPR11253] (ab157197)All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/datasheet/images/157/ab157197/DCTN5-Primary-antibodies-ab157197-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/datasheet/images/157/ab157197/DCTN5-Primary-antibodies-ab157197-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN5 antibody [EPR11253] (ab157197)Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution. -
![Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)](https://www.abcam.com/ps/datasheet/images/157/ab157197/DCTN5-Primary-antibodies-ab157197-5.jpg)
Immunoprecipitation - Anti-DCTN5 antibody [EPR11253] (ab157197)Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.
Protocols
References
ab157197 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"