Key features and details
- Rabbit polyclonal to DCTN6/WS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DCTN6/WS3 antibody
See all DCTN6/WS3 primary antibodies
DescriptionRabbit polyclonal to DCTN6/WS3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
Recombinant full length protein corresponding to Human DCTN6/WS3 aa 1-190.
MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVI GEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMK MGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLR RVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN
Database link: O00399
- WB: Mouse lung, stomach, thymus and gonadal lysates; A549 and U-251 MG whole cell lysates; Rat brain lysate. IHC-P: Human gastric cancer and kidney tissues. IP: A549 whole cell lysate.
This product was previously labelled as DCTN6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 21 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
Sequence similaritiesBelongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DCTN6 antibody
- DCTN6_HUMAN antibody
- Dynactin 6 antibody
All lanes : Anti-DCTN6/WS3 antibody (ab230014) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse gonadal lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : Mouse thymus lysate
Lane 6 : Rat brain lysate
Lane 7 : Mouse stomach lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Paraffin-embedded human kidney tissue staned for DCTN6/WS3 using ab230014 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for DCTN6/WS3 using ab230014 at 1/100 dilution in immunohistochemical analysis.
DCTN6/WS3 was immunoprecipitated from 500 µg A549 (human lung carcinoma cell line) whole cell lysate with 1 µg of ab230014.
Lane 1: 1 µg rabbit monoclonal IgG instead of ab230014 in A549 whole cell lysate.
Lane 2: ab230014 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate 20 µg (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230014 has not yet been referenced specifically in any publications.