Key features and details
- Rabbit polyclonal to DCTN6/WS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DCTN6/WS3 antibody
See all DCTN6/WS3 primary antibodies
DescriptionRabbit polyclonal to DCTN6/WS3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant full length protein corresponding to Human DCTN6/WS3 aa 1-190.
MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVI GEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMK MGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLR RVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN
Database link: O00399
- WB: A549 and U-251 MG whole cell lysates. IP: A549 whole cell lysate. IHC-P: Human gastric cancer and kidney tissues.
This product was previously labelled as DCTN6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 21 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
Sequence similaritiesBelongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DCTN6 antibody
- DCTN6_HUMAN antibody
- Dynactin 6 antibody
All lanes : Anti-DCTN6/WS3 antibody (ab230038) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Paraffin-embedded human gastric cancer tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.
DCTN6/WS3 was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with 4 µg of ab230038. Western blot was performed from the immunoprecipitate using ab230038. A HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg Rabbit monoclonal IgG instead of ab230038 in A549 whole cell lysate.
Lane 2: ab230038 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230038 has not yet been referenced specifically in any publications.