Product nameAnti-DCTN6/WS3 antibody
See all DCTN6/WS3 primary antibodies
DescriptionRabbit polyclonal to DCTN6/WS3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant full length protein corresponding to Human DCTN6/WS3 aa 1-190.
MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVI GEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMK MGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLR RVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN
Database link: O00399
- WB: A549 and U-251 MG whole cell lysates. IP: A549 whole cell lysate. IHC-P: Human gastric cancer and kidney tissues.
This product was previously labelled as DCTN6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 21 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
Sequence similaritiesBelongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- DCTN6 antibody
- DCTN6_HUMAN antibody
- Dynactin 6 antibody
All lanes : Anti-DCTN6/WS3 antibody (ab230038) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Paraffin-embedded human gastric cancer tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.
DCTN6/WS3 was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with 4 µg of ab230038. Western blot was performed from the immunoprecipitate using ab230038. A HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg Rabbit monoclonal IgG instead of ab230038 in A549 whole cell lysate.
Lane 2: ab230038 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate 20 µg (Input).
ab230038 has not yet been referenced specifically in any publications.