Overview

  • Product name

  • Description

    Rabbit polyclonal to DCTN6/WS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human DCTN6/WS3 aa 1-190.
    Sequence:

    MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVI GEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMK MGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLR RVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN


    Database link: O00399

  • Positive control

    • WB: A549 and U-251 MG whole cell lysates. IP: A549 whole cell lysate. IHC-P: Human gastric cancer and kidney tissues.
  • General notes

     This product was previously labelled as DCTN6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 21 kDa.
IP 1/200 - 1/2000.
IHC-P 1/20 - 1/200.

Target

Images

  • All lanes : Anti-DCTN6/WS3 antibody (ab230038) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Paraffin-embedded human gastric cancer tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for DCTN6/WS3 using ab230038 at 1/100 dilution in immunohistochemical analysis.

  • DCTN6/WS3 was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with 4 µg of ab230038. Western blot was performed from the immunoprecipitate using ab230038. A HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.

    Lane 1: 1 µg Rabbit monoclonal IgG instead of ab230038 in A549 whole cell lysate. 
    Lane 2: ab230038 IP in A549 whole cell lysate. 
    Lane 3: A549 whole cell lysate 20 µg (Input). 

References

ab230038 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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