Overview

  • Product name

  • Description

    Rabbit polyclonal to DCUN1D1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human DCUN1D1 (internal sequence). The exact sequence is proprietary.
    Database link: Q96GG9

  • Positive control

    • WB: A431, Jurkat and Raji whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.

Target

  • Relevance

    DCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.
  • Database links

  • Alternative names

    • DCN1 defective in cullin neddylation 1 domain containing 1 antibody
    • DCN1 like protein 1 antibody
    • DCNL1 antibody
    • DCUN1 domain containing protein 1 antibody
    • DCUN1L1 antibody
    • Defective in cullin neddylation protein 1 like protein 1 antibody
    • RP 42 antibody
    • RP42 antibody
    • RP42 homolog antibody
    • SCCRO antibody
    • SCRO antibody
    • Squamous cell carcinoma related oncogene antibody
    • Tes 3 antibody
    • Tes3 antibody
    see all

Images

  • All lanes : Anti-DCUN1D1 antibody (ab227547) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 30 kDa



    12% SDS-PAGE

References

ab227547 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227547.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up