Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab99506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa.|
RelevanceDCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.
- DCN1 defective in cullin neddylation 1 domain containing 1 antibody
- DCN1 like protein 1 antibody
- DCNL1 antibody
ab99506 (5µg/ml) staining of paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Tissue treated by steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-DCUN1D1 antibody (ab99506) at 0.5 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 1 hour
ab99506 has not yet been referenced specifically in any publications.