Anti-DCXR antibody (ab154290)
Overview
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Product name
Anti-DCXR antibody
See all DCXR primary antibodies -
Description
Rabbit polyclonal to DCXR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-244 of Human DCXR (UniProt: Q7Z4W1).
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Positive control
- HepG2 whole cell lysate; Human H661 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. -
Tissue specificity
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis. -
Involvement in disease
Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol. - Information by UniProt
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Database links
- Entrez Gene: 526937 Cow
- Entrez Gene: 51181 Human
- Entrez Gene: 67880 Mouse
- Entrez Gene: 171408 Rat
- Omim: 608347 Human
- SwissProt: Q1JP75 Cow
- SwissProt: Q7Z4W1 Human
- SwissProt: Q91X52 Mouse
see all -
Alternative names
- Carbonyl reductase 2 antibody
- Carbonyl reductase II antibody
- DCR antibody
see all
Images
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Western blot - Anti-DCXR antibody (ab154290)Anti-DCXR antibody (ab154290) at 1/1000 dilution + HepG2 whole cell
lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCXR antibody (ab154290)Immunohistochemical analysis of paraffin-embedded Human H661 xenograft tissue labeling DCXR with ab154290 at 1/500 dilution.
Protocols
References
ab154290 has not yet been referenced specifically in any publications.
