Anti-DDAH1 antibody (ab2231)
Key features and details
- Goat polyclonal to DDAH1
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DDAH1 antibody
See all DDAH1 primary antibodies -
Description
Goat polyclonal to DDAH1 -
Host species
Goat -
Tested applications
Suitable for: ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
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Positive control
- WB: HepG2 and LnCaP cell lysates. Human cerebellum, kidney and testes. ICC: HeLa and U20S cells Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
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| WB | (3) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
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ICC
Use a concentration of 10 µg/ml. |
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Flow Cyt
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
Tissue specificity
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
Sequence similarities
Belongs to the DDAH family. - Information by UniProt
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Database links
- Entrez Gene: 23576 Human
- Omim: 604743 Human
- SwissProt: P56965 Cow
- SwissProt: O94760 Human
- Unigene: 379858 Human
- Unigene: 713411 Human
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Alternative names
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
Images
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Flow Cytometry - Anti-DDAH1 antibody (ab2231)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 1ug/ml. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry - Anti-DDAH1 antibody (ab2231)Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody. -
Immunocytochemistry - Anti-DDAH1 antibody (ab2231)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody. -
Western blot - Anti-DDAH1 antibody (ab2231)All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml
Lane 1 : Human cerebellum
Lane 2 : Human kidney
Lane 3 : Human testes
Predicted band size: 31 kDa -
Western blot - Anti-DDAH1 antibody (ab2231)All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : LnCaP cell lysate
Predicted band size: 31 kDa
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab2231 has been referenced in 15 publications.
- Ding X et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
- Zhu ZD et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
- Joris V et al. MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the Endothelium. Arterioscler Thromb Vasc Biol 38:2345-2357 (2018). PubMed: 29976767
- Balasubramanian V et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
- Hulin JA et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
