• Product name
  • Description
    Goat polyclonal to DDAH1
  • Host species
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human DDAH1 aa 273-285 (C terminal).


    Database link: O94760
    (Peptide available as ab99047)


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab2231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 24663083
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human DDAH1 peptide (ab99047).


  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH I antibody
    • DDAH-1 antibody
    • DDAH1 antibody
    • DDAH1_HUMAN antibody
    • DDAHI antibody
    • Dimethylargininase 1 antibody
    • Dimethylargininase-1 antibody
    • Dimethylarginine dimethylaminohydrolase 1 antibody
    • N(G) antibody
    • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
    • NG NG dimethylarginine dimethylaminohydrolase antibody
    see all


  • Anti-DDAH1 antibody (ab2231) at 0.100000001490116 µg/ml + Human kidney lysate at 35 µg

    Predicted band size: 31 kDa

  • ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Balasubramanian V  et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB . Read more (PubMed: 29263339) »
  • Hulin JA  et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). Read more (PubMed: 29070803) »
See all 12 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Mouse Tissue lysate - whole (Brain (Hippocampus))
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Brain (Hippocampus)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

圭一 角山

Verified customer

Submitted Nov 25 2015

Western blot
Mouse Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 09 2010

Western blot
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Richard D'Mello

Verified customer

Submitted Apr 14 2009


According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negative control is also showing a band at the same size. We do not have the peptide here for them to try but I will ask the originator of the antibody if they have the blocking peptide available. It would help if the researcher could contact us directly and provide a blot image. Are they just loading BSA in their negative control lane? Please ask them to contact us by e-mailing technical@abcam.com and attaching a blot image to the e-mail.

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