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    ddah1-antibody-ab2231.pdf

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Neuroscience Neurotransmission Nitric Oxide NOS
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Anti-DDAH1 antibody (ab2231)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (14)

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Western blot - Anti-DDAH1 antibody (ab2231)

    Key features and details

    • Goat polyclonal to DDAH1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    Protein
    Product image
    Recombinant Human DDAH1 protein (ab99863)

    View more associated products

    Overview

    • Product name

      Anti-DDAH1 antibody
      See all DDAH1 primary antibodies
    • Description

      Goat polyclonal to DDAH1
    • Host species

      Goat
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Cow
    • Immunogen

      Synthetic peptide corresponding to Human DDAH1 aa 273-285 (C terminal).
      Sequence:

      TCCSVLINKKVDS


      Database link: O94760
      (Peptide available as ab99047)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Nitric Oxide
      • NOS
      • Cardiovascular
      • Vasculature
      • Endothelium

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human DDAH1 protein (ab99863)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2231 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (3)
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
    • Tissue specificity

      Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
    • Sequence similarities

      Belongs to the DDAH family.
    • Target information above from: UniProt accession O94760 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23576 Human
      • Omim: 604743 Human
      • SwissProt: P56965 Cow
      • SwissProt: O94760 Human
      • Unigene: 379858 Human
      • Unigene: 713411 Human
      • Alternative names

        • DDAH antibody
        • DDAH I antibody
        • DDAH-1 antibody
        • DDAH1 antibody
        • DDAH1_HUMAN antibody
        • DDAHI antibody
        • Dimethylargininase 1 antibody
        • Dimethylargininase-1 antibody
        • Dimethylarginine dimethylaminohydrolase 1 antibody
        • N(G) antibody
        • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
        • NG NG dimethylarginine dimethylaminohydrolase antibody
        see all

      Images

      • Western blot - Anti-DDAH1 antibody (ab2231)
        Western blot - Anti-DDAH1 antibody (ab2231)
        Anti-DDAH1 antibody (ab2231) at 0.100000001490116 µg/ml + Human kidney lysate at 35 µg

        Predicted band size: 31 kDa

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (14)

      Publishing research using ab2231? Please let us know so that we can cite the reference in this datasheet.

      ab2231 has been referenced in 14 publications.

      • Zhu ZD  et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
      • Joris V  et al. MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the Endothelium. Arterioscler Thromb Vasc Biol 38:2345-2357 (2018). PubMed: 29976767
      • Balasubramanian V  et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
      • Hulin JA  et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
      • Mookerjee RP  et al. Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension. J Hepatol 62:325-31 (2015). WB, IHC ; Human . PubMed: 25152204
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-DDAH1 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain (Hippocampus))
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      Brain (Hippocampus)
      Blocking step
      Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
      Read More

      圭一 角山

      Verified customer

      Submitted Nov 25 2015

      Western blot abreview for Anti-DDAH1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (hippocampus)
      Loading amount
      5 µg
      Specification
      hippocampus
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 09 2010

      Western blot abreview for Anti-DDAH1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Hippocampus lysate)
      Loading amount
      40 µg
      Specification
      Hippocampus lysate
      Gel Running Conditions
      Reduced Denaturing (12% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Mr. Richard D'Mello

      Verified customer

      Submitted Apr 14 2009

      Question

      Hi! So, we had a customer call today who is using ab 2231 in both human and rat aortic homogenate. He has loaded from 100 ugs to 1 mg of protein per lane diluted the antibody 5 ugs/ml and incubated overnight. He sees a band at 32kDa but nothing at 40 kDa. It says on the datasheet that the predicted MW is 33 kDa but runs at 40 kDa do you have the peptide available for them to run a blocking study? The other issue is that they see this band in their negative control lane (with BSA). Otherwise, this looks to be an issue with the antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 20 2003

      Answer

      According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negative control is also showing a band at the same size. We do not have the peptide here for them to try but I will ask the originator of the antibody if they have the blocking peptide available. It would help if the researcher could contact us directly and provide a blot image. Are they just loading BSA in their negative control lane? Please ask them to contact us by e-mailing technical@abcam.com and attaching a blot image to the e-mail.

      Read More

      Abcam Scientific Support

      Answered on Jun 20 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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