Product nameAnti-DDAH1 antibody
See all DDAH1 primary antibodies
DescriptionRabbit polyclonal to DDAH1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human DDAH1 aa 175-225. The exact sequence is proprietary.
Database link: O94760
- WB: HeLa and HEK-293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 31 kDa.|
FunctionHydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue specificityDetected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Sequence similaritiesBelongs to the DDAH family.
- Information by UniProt
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
All lanes : Anti-DDAH1 antibody (ab264400) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264400 has not yet been referenced specifically in any publications.