Recombinant
RabMAb

Anti-DDAH1 antibody [EPR13921] (ab181859)

Overview

  • Product name
    Anti-DDAH1 antibody [EPR13921]
    See all DDAH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13921] to DDAH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DDAH1 aa 1-100. The exact sequence is proprietary.
    Database link: O94760

  • Positive control
    • 293 and HepG2 cell lysates; Human hepatocellular carcinoma tissue; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13921
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40.

Target

  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH I antibody
    • DDAH-1 antibody
    • DDAH1 antibody
    • DDAH1_HUMAN antibody
    • DDAHI antibody
    • Dimethylargininase 1 antibody
    • Dimethylargininase-1 antibody
    • Dimethylarginine dimethylaminohydrolase 1 antibody
    • N(G) antibody
    • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
    • NG NG dimethylarginine dimethylaminohydrolase antibody
    see all

Images

  • Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling DDAH1 with ab181859 at 1/30 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling DDAH1 with ab181859 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)

References

ab181859 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab181859.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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