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Rabbit monoclonal [EPR13921] to DDAH1
Synthetic peptide within Human DDAH1 aa 1-100. The exact sequence is proprietary.
Database link: O94760
293 and HepG2 cell lysates; Human hepatocellular carcinoma tissue; 293 cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Belongs to the DDAH family.
Information by UniProt
DDAH I antibody
Immunoprecipitation - Anti-DDAH1 antibody [EPR13921] (ab181859)
Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
Flow Cytometry - Anti-DDAH1 antibody [EPR13921] (ab181859)
Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling DDAH1 with ab181859 at 1/30 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH1 antibody [EPR13921] (ab181859)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling DDAH1 with ab181859 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Western blot - Anti-DDAH1 antibody [EPR13921] (ab181859)
All lanes :
Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/5000 dilution
Lane 1 :
293 cell lysate
Lane 2 :
HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"