Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human DDAH2 peptide (ab22866).

Please note that DDAH2, according to NP_039268, has a consensus glycosylation site. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

IHC-FoFr Use at an assay dependent concentration. PubMed: 21408057

Target

  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH II antibody
    • DDAH-2 antibody
    • DDAH2 antibody
    • DDAH2_HUMAN antibody
    • DDAHII antibody
    • Dimethylargininase 2 antibody
    • Dimethylargininase-2 antibody
    • Dimethylarginine dimethylaminohydrolase 2 antibody
    • Dimethylarginine dimethylaminohydrolase II antibody
    • G6a antibody
    • N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 antibody
    • NG dimethylarginine dimethylamino hydrolase homolog antibody
    • NG30 antibody
    • OTTHUMP00000029307 antibody
    • OTTHUMP00000029310 antibody
    • OTTHUMP00000174488 antibody
    • OTTHUMP00000174489 antibody
    • Protein G6a antibody
    • S phase protein antibody
    • S-phase protein antibody
    see all

Images

  • Anti-DDAH2 antibody (ab1383) at 0.1 µg/ml + Human Lung lysate at 35 µg

    Predicted band size: 30 kDa

  • Anti-DDAH2 antibody (ab1383) at 1 µg/ml + Human Heart extracts at 35 µg

    Predicted band size: 30 kDa

References

This product has been referenced in:
  • Mookerjee RP  et al. Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension. J Hepatol 62:325-31 (2015). WB ; Human . Read more (PubMed: 25152204) »
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr ; Rat . Read more (PubMed: 24663083) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
40 µg
Specification
Hela
Blocking step
Milk as blocking agent for 50 minute(s) · Concentration: 4% · Temperature: RT°C

Frau Dr. Marion Meixner

Verified customer

Submitted Jun 23 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus)
Loading amount
40 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Richard D'Mello

Verified customer

Submitted Apr 14 2009

Answer

Thank you for your enquiry. This is a well sold product and has been around for quite a while and we are currently selling the third batch. Interestingly, the very first batch did react with a 40kDa band in lysates of Mouse Heart. This would be consistent with the observed 38kDa band. I have to say that this result was obtained at 2ug/ml and possibly after overnight incubation. The second batch (different goat) however, showed a 28kDa band in both Human Heart and Mouse Heart. This was obtained at 1ug/ml after only 1 hr incubation. The same is true for the current batch on sale (from a third goat). It is most probable that the Hypertension research 2005 28: 255-262 used an antibody from the first batch. However, please rest assured that the current info on our datasheet is accurate and states the latest results for the batch testing. Also, according to SwissProt, the molecular weight should be around 30kDa.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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