Key features and details
- Rabbit polyclonal to DDAH2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-DDAH2 antibody
See all DDAH2 primary antibodies
DescriptionRabbit polyclonal to DDAH2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment (His-tag) corresponding to Human DDAH2 aa 31-265. (Expressed in E.coli).
AGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAV IQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVL FTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGG PRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGV PPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSEL
Database link: O95865
- WB: Recombinant human DDAH2 protein; Pig brain lysate; MCF7 cell lysate. IHC-P: Human prostate, brain, colorectal cancer, glioma and stomach cancer tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232694 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 30 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionHydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue specificityDetected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Sequence similaritiesBelongs to the DDAH family.
- Information by UniProt
- DDAH antibody
- DDAH II antibody
- DDAH-2 antibody
Paraffin-embedded human prostate tissue stained for DDAH2 using ab232694 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human brain tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-DDAH2 antibody (ab232694) at 2 µg/ml + Recombinant human DDAH2 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
All lanes : Anti-DDAH2 antibody (ab232694) at 2 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : Pig brain lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Paraffin-embedded human colorectal cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach cancer tissue stained for DDAH2 using ab232694 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232694 has not yet been referenced specifically in any publications.