Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15508(B)] to DDAH2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-DDAH2 antibody [EPR15508(B)]
See all DDAH2 primary antibodies
DescriptionRabbit monoclonal [EPR15508(B)] to DDAH2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DDAH2 aa 1-100. The exact sequence is proprietary.
Database link: O95865
- MCF7, Human fetal brain, Human fetal heart and Human testis lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|IP||1/40 - 1/60.|
FunctionHydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue specificityDetected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Sequence similaritiesBelongs to the DDAH family.
- Information by UniProt
- DDAH antibody
- DDAH II antibody
- DDAH-2 antibody
Western blot analysis of immunoprecipitation pellet from Human fetal brain tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184166 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes : Anti-DDAH2 antibody [EPR15508(B)] (ab184166) at 1/10000 dilution
Lane 1 : MCF7 cell line lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human testis tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
ab184166 has been referenced in 2 publications.
- Badran M et al. Alpha Lipoic Acid Improves Endothelial Function and Oxidative Stress in Mice Exposed to Chronic Intermittent Hypoxia. Oxid Med Cell Longev 2019:4093018 (2019). PubMed: 31093313
- Wang H et al. DDAH1 plays dual roles in PM2.5 induced cell death in A549 cells. Biochim Biophys Acta 1860:2793-801 (2016). PubMed: 26996393