Anti-DDB1 antibody (ab245579)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DDB1 antibody
See all DDB1 primary antibodies -
Description
Rabbit polyclonal to DDB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Orangutan
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Immunogen
Synthetic peptide within Human DDB1 aa 1100-1140 (C terminal). The exact sequence is proprietary.
Database link: Q13289 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and mouse CT26 colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245579 was affinity purified using an epitope specific to DDB1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 127 kDa. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the DDB1 family. -
Post-translational
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localization
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 100173610 Orangutan
- Entrez Gene: 64470 Rat
- Entrez Gene: 399026 Xenopus laevis
- Omim: 600045 Human
see all -
Alternative names
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)Formalin-fixed, paraffin-embedded mouse CT26 colon carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Western blot - Anti-DDB1 antibody (ab245579)All lanes : Anti-DDB1 antibody (ab245579) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 127 kDa
Exposure time: 10 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-DDB1 antibody (ab245579)DDB1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245579 used for IP at 3 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab245579 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab245579 has not yet been referenced specifically in any publications.
