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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-DDB1 antibody (ab245579)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)
  • Western blot - Anti-DDB1 antibody (ab245579)
  • Immunoprecipitation - Anti-DDB1 antibody (ab245579)

Key features and details

  • Rabbit polyclonal to DDB1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human DDB1 protein (ab114333)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DDB1 antibody
    See all DDB1 primary antibodies
  • Description

    Rabbit polyclonal to DDB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Orangutan
  • Immunogen

    Synthetic peptide within Human DDB1 aa 1100-1140 (C terminal). The exact sequence is proprietary.
    Database link: Q13289

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and mouse CT26 colon carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245579 was affinity purified using an epitope specific to DDB1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human DDB1 protein (ab114333)
  • Related Products

    • Recombinant Human DDB1 protein (ab114333)

Applications

Our Abpromise guarantee covers the use of ab245579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 127 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the DDB1 family.
  • Post-translational
    modifications

    Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • Cellular localization

    Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Target information above from: UniProt accession Q16531 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 511951 Cow
    • Entrez Gene: 100290337 Human
    • Entrez Gene: 1642 Human
    • Entrez Gene: 13194 Mouse
    • Entrez Gene: 100173610 Orangutan
    • Entrez Gene: 64470 Rat
    • Entrez Gene: 399026 Xenopus laevis
    • Omim: 600045 Human
    • SwissProt: A1A4K3 Cow
    • SwissProt: Q16531 Human
    • SwissProt: Q3U1J4 Mouse
    • SwissProt: Q5R649 Orangutan
    • SwissProt: Q9ESW0 Rat
    • SwissProt: Q6P6Z0 Xenopus laevis
    • Unigene: 290758 Human
    • Unigene: 289915 Mouse
    • Unigene: 466856 Mouse
    • Unigene: 8402 Rat
    • Unigene: 23906 Xenopus laevis
    see all
  • Alternative names

    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab245579)

    Formalin-fixed, paraffin-embedded mouse CT26 colon carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-DDB1 antibody (ab245579)
    Western blot - Anti-DDB1 antibody (ab245579)
    All lanes : Anti-DDB1 antibody (ab245579) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 127 kDa


    Exposure time: 10 seconds


    Prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-DDB1 antibody (ab245579)
    Immunoprecipitation - Anti-DDB1 antibody (ab245579)

    DDB1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    ab245579 used for IP at 3 µg per reaction. For WB 0.4 µg/ml.

    Lane 1: ab245579 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 30 seconds.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245579? Please let us know so that we can cite the reference in this datasheet.

    ab245579 has not yet been referenced specifically in any publications.

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