Key features and details
- Rabbit polyclonal to DDB1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DDB1 antibody
See all DDB1 primary antibodies
DescriptionRabbit polyclonal to DDB1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Orangutan
Synthetic peptide within Human DDB1 aa 1100-1140 (C terminal). The exact sequence is proprietary.
Database link: Q13289
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and mouse CT26 colon carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245579 was affinity purified using an epitope specific to DDB1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 127 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the DDB1 family.
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
Cellular localizationCytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
- Information by UniProt
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse CT26 colon carcinoma tissue stained for DDB1 with ab245579 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-DDB1 antibody (ab245579) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 127 kDa
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
DDB1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245579 used for IP at 3 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab245579 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245579 has not yet been referenced specifically in any publications.