Anti-DDB1 antibody (ab9194)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-DDB1 antibody
See all DDB1 primary antibodies -
DescriptionGoat polyclonal to DDB1
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Host speciesGoat
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig, Xenopus laevis
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
Sequence:C-DLIKVVEELTRIH
(Peptide available asab23152) -
Positive control
- WB: HeLa, Jurkat and NSO (mouse) cell lysates.
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General notes
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Primary antibody notesRecognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).Can be blocked with Human DDB1 peptide (ab23152). | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 17635991 |
Target
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FunctionRequired for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the DDB1 family.
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Post-translational
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localizationCytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
- Information by UniProt
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Database links
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Entrez Gene: 399026 Xenopus laevis
- Omim: 600045 Human
- SwissProt: A1A4K3 Cow
see all -
Alternative names
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
Images
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Western blot - Anti-DDB1 antibody (ab9194)Goat polyclonal to DDB-1 (1
Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.µ g/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.
Datasheets and documents
References
This product has been referenced in:
- Pines A et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . Read more (PubMed: 29531219) »
- Pines A et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . Read more (PubMed: 23045548) »
