Overview

  • Product name
    Anti-DDB1 antibody
    See all DDB1 primary antibodies
  • Description
    Goat polyclonal to DDB1
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Xenopus laevis
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
    Sequence:

    C-DLIKVVEELTRIH


    (Peptide available as ab23152)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • WB: HeLa, Jurkat and NSO (mouse) cell lysates.
  • General notes


    Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).Can be blocked with Human DDB1 peptide (ab23152).
ICC/IF Use at an assay dependent dilution. PubMed: 17635991

Target

  • Function
    Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the DDB1 family.
  • Post-translational
    modifications
    Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • Cellular localization
    Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

Images

  • Western blot - Anti-DDB1 antibody (ab9194)
    Western blot - Anti-DDB1 antibody (ab9194)

    Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.

    Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Pines A  et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . Read more (PubMed: 29531219) »
  • Pines A  et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . Read more (PubMed: 23045548) »

See all 8 Publications for this product

Publishing research using ab9194? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-DDB1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (st II-III oocytes)
Gel Running Conditions
Reduced Denaturing (4-20% precast)
Loading amount
20 cells
Specification
st II-III oocytes
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
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Username

Ms. Samantha Jeschonek

Verified customer

Submitted Feb 22 2017

Western blot abreview for Anti-DDB1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Interphase Egg extract)
Loading amount
40 µg
Specification
Interphase Egg extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Nov 08 2012

Is this antibody ( ab9194) suitable for immunoprecipitation ?

This antibody has never been tested in immunoprecipitation, therefore we can't guarantee results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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