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    ddb1-antibody-ab9194.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-DDB1 antibody (ab9194)

  • Datasheet
Reviews (2)Q&A (1)References (10)

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Western blot - Anti-DDB1 antibody (ab9194)

    Key features and details

    • Goat polyclonal to DDB1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-DDB1 antibody [EPR6089] (ab109027)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-DDB1 antibody
      See all DDB1 primary antibodies
    • Description

      Goat polyclonal to DDB1
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
      Sequence:

      C-DLIKVVEELTRIH


      (Peptide available as ab23152)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes


      Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody notes

      Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • DNA Damage Recognition

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human DDB1 protein (ab114333)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9194 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Cow
    Dog
    Application Abreviews Notes
    WB (2)
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the DDB1 family.
    • Post-translational
      modifications

      Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
    • Cellular localization

      Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
    • Target information above from: UniProt accession Q16531 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 511951 Cow
      • Entrez Gene: 13194 Mouse
      • SwissProt: A1A4K3 Cow
      • SwissProt: Q3U1J4 Mouse
      • Unigene: 289915 Mouse
      • Unigene: 466856 Mouse
      • Alternative names

        • Damage specific DNA binding protein 1 antibody
        • Damage-specific DNA-binding protein 1 antibody
        • DDB 1 antibody
        • DDB p127 subunit antibody
        • Ddb1 antibody
        • DDB1_HUMAN antibody
        • DDBa antibody
        • DNA damage binding protein 1 antibody
        • DNA damage-binding protein 1 antibody
        • DNA damage-binding protein a antibody
        • HBV X-associated protein 1 antibody
        • UV damaged DNA binding factor antibody
        • UV damaged DNA binding protein 1 antibody
        • UV DDB 1 antibody
        • UV DDB1 antibody
        • UV-damaged DNA-binding factor antibody
        • UV-damaged DNA-binding protein 1 antibody
        • UV-DDB 1 antibody
        • X associated protein 1 antibody
        • XAP 1 antibody
        • XAP-1 antibody
        • XAP1 antibody
        • Xeroderma pigmentosum group E complementing protein antibody
        • Xeroderma pigmentosum group E-complementing protein antibody
        • XPCe antibody
        • XPE antibody
        • XPE BF antibody
        • XPE binding factor antibody
        • XPE-BF antibody
        • XPE-binding factor antibody
        see all

      Images

      • Western blot - Anti-DDB1 antibody (ab9194)
        Western blot - Anti-DDB1 antibody (ab9194)

        Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.

        Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (10)

      Publishing research using ab9194? Please let us know so that we can cite the reference in this datasheet.

      ab9194 has been referenced in 10 publications.

      • Yang C  et al. Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4DCAF4 E3 ligases to inhibit colorectal cancer cell growth. Int J Biol Sci 16:1059-1070 (2020). PubMed: 32140073
      • Sun H  et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
      • Pines A  et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . PubMed: 29531219
      • Pines A  et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . PubMed: 23045548
      • Anvar SY  et al. Deregulation of the ubiquitin-proteasome system is the predominant molecular pathology in OPMD animal models and patients. Skelet Muscle 1:15 (2011). ICC/IF ; Mouse . PubMed: 21798095
      • Leung-Pineda V  et al. DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress. Cancer Res 69:2630-7 (2009). WB ; Human . PubMed: 19276361
      • Alekseev S  et al. Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage. Mol Cell Biol 28:7402-13 (2008). WB, ICC/IF ; Mouse . PubMed: 18936169
      • Luijsterburg MS  et al. Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC. J Cell Sci 120:2706-16 (2007). WB, ICC/IF ; Human . PubMed: 17635991
      • Westbrook TF  et al. A genetic screen for candidate tumor suppressors identifies REST. Cell 121:837-48 (2005). PubMed: 15960972
      • Ulane CM  et al. STAT3 ubiquitylation and degradation by mumps virus suppress cytokine and oncogene signaling. J Virol 77:6385-93 (2003). WB . PubMed: 12743296

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-DDB1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Xenopus laevis Cell lysate - whole cell (st II-III oocytes)
      Gel Running Conditions
      Reduced Denaturing (4-20% precast)
      Loading amount
      20 cells
      Specification
      st II-III oocytes
      Blocking step
      Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
      Read More

      Ms. Samantha Jeschonek

      Verified customer

      Submitted Feb 22 2017

      Western blot abreview for Anti-DDB1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Xenopus laevis Cell lysate - whole cell (Interphase Egg extract)
      Loading amount
      40 µg
      Specification
      Interphase Egg extract
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 08 2012

      Question

      Is this antibody ( ab9194) suitable for immunoprecipitation ?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 24 2002

      Answer

      This antibody has never been tested in immunoprecipitation, therefore we can't guarantee results.

      Read More

      Abcam Scientific Support

      Answered on Jun 25 2002

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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