Anti-DDB1 antibody (ab9194)
Key features and details
- Goat polyclonal to DDB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-DDB1 antibody
See all DDB1 primary antibodies -
Description
Goat polyclonal to DDB1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
Sequence:C-DLIKVVEELTRIH
(Peptide available asab23152) -
Positive control
- WB: HeLa lysate, HepG2 lysate, Jurkat lysate, NIH3T3 lysate and NSO lysate. IHC-P: Human cortex tissue.
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General notes
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 0.01 - 2 µg/ml. Predicted molecular weight: 127 kDa.
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.01 - 2 µg/ml. Predicted molecular weight: 127 kDa. 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the DDB1 family. -
Post-translational
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localization
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: A1A4K3 Cow
- SwissProt: Q16531 Human
see all -
Alternative names
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
Images
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All lanes : Anti-DDB1 antibody (ab9194) at 1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 127 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
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All lanes : Anti-DDB1 antibody (ab9194) at 0.01 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NSO (Murine myeloma cells) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 127 kDa
Observed band size: 140,150 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
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Left panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue labelling DDB1 with ab9194 at 4µg/ml. Heat mediated antigen retrieval with citrate buffer pH 6, HRP-staining.
Right panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue with no primary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab9194 has been referenced in 11 publications.
- Yang C et al. Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4DCAF4 E3 ligases to inhibit colorectal cancer cell growth. Int J Biol Sci 16:1059-1070 (2020). PubMed: 32140073
- Tong X et al. DDB1 E3 ligase controls dietary fructose-induced ChREBPa stabilization and liver steatosis via CRY1. Metabolism 107:154222 (2020). PubMed: 32246987
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Pines A et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . PubMed: 29531219
- Pines A et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . PubMed: 23045548