Overview

  • Product name

    Anti-DDB1 antibody - C-terminal
    See all DDB1 primary antibodies
  • Description

    Rabbit polyclonal to DDB1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human DDB1 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q16531

  • Positive control

    • WB: A549, H1299, HEK-293T, Neuro-2a, GL261, C8D30, PC-12, HCT 116 and MCF7 whole cell extracts. ICC/IF: HeLa cells. IHC-P: U373 xenograft tissue; mouse heart, hind brain and duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 127 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the DDB1 family.
  • Post-translational
    modifications

    Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • Cellular localization

    Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

Images

  • All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell extracts
    Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell extracts
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extracts
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 127 kDa



    7.5% SDS PAGE

  • Paraffin embedded mouse duodenum tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DDB1 (green) using ab226968 (1/500 dilution) in ICC/IF. HeLa cells were fixed in ice-cold methanol for 5 minutes. Counterstain: Hoechst 33343 (blue).

  • All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/10000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : DDB1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 127 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
    Lane 2 : GL261 whole cell extracts
    Lane 3 : C8D30 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 127 kDa



    7.5% SDS PAGE

  • Anti-DDB1 antibody - C-terminal (ab226968) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 127 kDa



    7.5% SDS PAGE

  • Paraffin embedded mouse hind brain tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.

  • Paraffin embedded mouse heart tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.

  • Paraffin embedded U373 (human) xenograft tissue stained for DDB1 with ab226968 (1/500 dilution) in immunohistochemical analysis.

References

ab226968 has not yet been referenced specifically in any publications.

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