Anti-DDB1 antibody [EPR6089] (ab109027)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-DDB1 antibody [EPR6089]
See all DDB1 primary antibodies -
DescriptionRabbit monoclonal [EPR6089] to DDB1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human DDB1 aa 1100-1200 (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2, HeLa, NIH3T3, and Human platelet lysates, Human breast tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: UV-treated HeLa
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 8.40 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6089
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/50 - 1/100. |
Target
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FunctionRequired for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the DDB1 family.
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Post-translational
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localizationCytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
- Information by UniProt
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Database links
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: Q16531 Human
- SwissProt: Q3U1J4 Mouse
- SwissProt: Q9ESW0 Rat
see all -
Alternative names
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)](https://www.abcam.com/ps/products/109/ab109027/Images/ab109027-221519-ab109027gr429056edited.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-DDB1 antibody [EPR6089] (ab109027)](https://www.abcam.com/ps/products/109/ab109027/Images/DDB1-Primary-antibodies-ab109027-3.JPG)
Western blot - Anti-DDB1 antibody [EPR6089] (ab109027)All lanes : Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 127 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)](https://www.abcam.com/ps/products/109/ab109027/Images/DDB1-Primary-antibodies-ab109027-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100. -
![Other - Anti-DDB1 antibody [EPR6089] (ab109027)](https://www.abcam.com/ps/products/109/ab109027/Images/DDB1-Primary-antibodies-ab109027-4.jpg)
Other - Anti-DDB1 antibody [EPR6089] (ab109027)
Protocols
References
This product has been referenced in:
- Lu H et al. Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nat Commun 8:2039 (2017). WB . Read more (PubMed: 29229926) »
- Li BT et al. TET1 inhibits cell proliferation by inducing RASSF5 expression. Oncotarget 8:86395-86409 (2017). WB . Read more (PubMed: 29156803) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"