Recombinant Anti-DDB1 antibody [EPR6089] (ab109027)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6089] to DDB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-DDB1 antibody [EPR6089]
See all DDB1 primary antibodies -
Description
Rabbit monoclonal [EPR6089] to DDB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, NIH3T3, and Human platelet lysates, Human breast tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: UV-treated HeLa
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6089 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/100. |
WB
1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the DDB1 family. -
Post-translational
modificationsUbiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localization
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: Q16531 Human
- SwissProt: Q3U1J4 Mouse
- SwissProt: Q9ESW0 Rat
see all -
Alternative names
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
Images
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All lanes : Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 127 kDa -
Immunocytochemistry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling DDB1 with ab109027 at 1/250 (8.9 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Confocal image showing nuclear and cytoplasmic staining in NIH/3T3 cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100. Antigen retrieval was heat mediated before commencing with IHC staining protocol.
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ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab109027 has been referenced in 13 publications.
- Okur MN et al. Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation. Nucleic Acids Res 48:2473-2485 (2020). PubMed: 31970402
- Lu H et al. DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response. Nucleic Acids Res 47:9467-9479 (2019). PubMed: 31396623
- Kim K et al. Disordered region of cereblon is required for efficient degradation by proteolysis-targeting chimera. Sci Rep 9:19654 (2019). PubMed: 31873151
- Zhang Y et al. Role of Damage DNA-Binding Protein 1 in Pancreatic Cancer Progression and Chemoresistance. Cancers (Basel) 11:N/A (2019). PubMed: 31842285
- Lu G et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy 15:1917-1934 (2019). PubMed: 30898011