Western blot abreview for Anti-DDDDK tag antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (hTERT-RPE1)
Loading amount
50000 cells
Specification
hTERT-RPE1
Treatment
transfected with flag tag plasmid 20hr
Gel Running Conditions
Reduced Denaturing (8 % acrylamid)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti Rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140, 102, 83 kDa

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Submitted Dec 14 2012

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