Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT-RPE1)
Specification
hTERT-RPE1
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.02% · Temperature: 18°C

Other product details

Dilution
1/100
Incubation time
45 minute(s) · Temperature: 18°C

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti Rabbit-Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
did not work with methanol fixation

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Submitted Dec 19 2012

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