This antibody was used in free floating coronal brain sections (30 microns) at room temperature. I used this antibody to stain for flag in a doubly transgenic mouse model (cell-type specific expression of myc or flag-tagged protein). Published data showed that myc and flag are expressed in separate cell types. I have seen this pattern of expression with 3 different flag antibodies in sections co-stained with myc antibody. Ab1162 had a very clear and bright expression, but it also overlapped completely with myc stained neurons.
Thank you for your valuable feedback. This antibody has not been previously tested in IHC-FoFr and so we cannot guarantee that it will work for this application. We welcome other abreviews to determine this.
Submitted Jul 28 2014
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